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The effect of arachidonic acid (AA) on intracellular Ca 2+ concentration ((Ca 2+ ) i ) in human osteoblasts MG63 was studied. AA caused a concentration-dependent increase in (Ca 2+ ) i , mainly due to inward Ca 2+ transport from extracellular environment. Moreover, AA in Ca 2+ -free medium...
The rate limiting steps in the conversion of 18-carbon unsaturated fatty acids to 20- and 22-carbon products are catalyzed by two desaturase enzymes (Δ5-desaturase and Δ6-desaturase) found within a lipid desaturase gene cluster. Careful examination of this cluster revealed the existence of a...
Introduction The lipid composition of spermatozoa plays an important role for successful fertilization.
We previously reported that prostaglandin D 2 (PGD 2 ) stimulates the induction of heat shock protein 27 (HSP27) in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether PGD 2 stimulates the phosphorylation of HSP27 in MC3T3-E1 cells exposed to heat shock. In the cultured...
Under physiological conditions, small amounts of free arachidonic acid (AA) are released from membrane phospholipids, and cyclooxygenase (COX) and acyl-CoA synthetase (ACS) competitively act on this fatty acid to form prostaglandins (PGs) and arachidonoyl-CoA (AA-CoA). In the present study, we...
Introduction Prostaglandins have an anabolic effect on bone. Possible mediation of this effect is via calcitriol. This study determines in vivo and in vitro effects of PGE 1 on calcitriol synthesis.
The oxidation of low-density lipoprotein (LDL) induces immunogenic epitopes, many of which are due to oxidatively modified phospholipids (oxPL). Lysophosphatidylcholine (lyso-PC) which is generated during LDL oxidation through the hydrolysis of oxPL by LDL-associated PAF-acetylhydrolase (PAF-AH)...
Prostaglandin E 2 (PGE 2 ) is bone-anabolic, i.e. stimulates bone formation and increases bone mass. In this study, we explored possible intracellular mechanisms of its increase of osteogenic cells in rat bone marrow. Adherent rat bone marrow cells were counted after 12–48 h or cultured for 21...
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