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Highlights ► The total, neutral and polar lipids were analyzed by cytomic approach. ► The differentiations of lipid profiles were analyzed by lipidomic approach. ► Twenty-two lipid molecules were discovered as biomarkers under NaCl stress. ► “Omics” studies provide better biological...
Highlights ► First report on aqueous two phase extraction (ATPE) for phytase purification. ► Highest phytase recovery of 98.5% in a single step ATPE method so far. ► Studies on chromatography vs. ATPE method for downstream processing of phytase. ► This study offers a viable option to the...
Highlights ► The mutant strain MECH-Y185/S226 with an improved endochitinase activity was obtained by the directed evolution. ► Amino acid residues Y185 and S226 were found to be altered in the mutant endochitinase. ► The mutant endochitinase has a higher thermal and pH stabilities. ► The...
Highlights ► Saccharomyces cerevisiae cells were immobilized within a plastic microchannel. ► A whole-cell microbioreactor was used for a continuous biotransformation. ► Several process parameters were evaluated regarding process productivity. ► The use of a microbioreactor enabled fast...
Highlights ► Flavin reductase DszD was purified from biodesulfurizing Mycobacterium goodii X7B. ► The DszD encoding gene dszD was amplified and expressed in Escherichia coli . ► Purified DszD binds FMN as the cofactor and can use FMN or FAD as the substrate. ► DszD could effectively...
Highlights ► 3-HP production from glycerol by K. pneumoniae is dependent on the activity of ALDH. ► KGSADH was the most efficient enzyme in 3-HP production among the ALDHs used here. ► To enhance 3-HP production, competing enzymes, DhaT and NADPH-HOR were eliminated. ► dhaT gene deletion...
Highlights ► A procedure of enzymatic desymmetrization of a prochiral diester was developed. ► An aqueous–organic phase containing phosphate buffer and MTBE was used. ► 95.6% ee of ( R )-3-MFG was obtained, together with the highest yield of 92.6%. ► Novozym 435 showed an excellent...
Highlights ► Laccase was immobilized on digested spent grain with a high yield. ► The kinetics of immobilized laccase followed the Michaelis–Menten model. ► The immobilized enzyme was stable during 10 cycles of reuse. ► The immobilized enzyme was stable during 48 days of storage.
Highlights ► Chitosan–ionic liquid–ferrocene/graphene composite modified glassy carbon electrode. ► Combined advantages of graphene, ferrocene and ionic liquid. ► Enhanced electrochemical response towards hemoglobin. ► Modified electrode has high electrocatalytic activity for...
Highlights ► A new lectin, named BfL, was purified from Bauhinia forficata seeds. ► Its hemagglutinating activity is inhibited by glycoproteins. ► The primary structure of BfL was determined. ► BfL increases the coagulation time and inhibits the platelet aggregation. ► BfL is the only...
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