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The partition of cellulase from Trichoderma viride in aqueous two-phase systems has been investigated with the purpose of designing a phase system for the bioconversion of cellulose. The partitioning of the enzyme in the two-phase systems composed of various water-soluble polymeric compounds, and...
The kinetics of the enzymic reaction of penicillin G acylase from a mutant of Escherichia coli ATCC 11105 in forward and reverse directions were studied and the kinetic constants determined. Results show that the enzyme is inhibited by excess substrate, penicillin G (Pen G), and by both products....
Immobilized lipase from Rhizomucor miehei (Lipozyme IM-20) was used for the synthesis of isobutyl isobutyrate by direct esterification of isobutyric acid and isobutyl alcohol. Response surface methodology based on a five-variable central composite rotatable design was used to determine the effect...