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A laboratory-scale hybrid anaerobic reactor, the bottom third of which was occupied by a sludge blanket, the upper two-thirds by submerged clay rings, was used for treatment of wash waters derived from the purification of olive oil in a new manufacturing process. The reactor was operated under...
The rate of mass transfer in the liquid-liquid extraction of α-amylase from an aqueous phase to a reversed micellar phase has been studied. A simple model for the mathematical representation of this system is discussed. Batch separation experiments at different total concentrations have been...
The production of leuconocin S, a bacteriocin from Leuconostoc paramesenteroides OX, was examined during batch fermentations at pH 6·0, 6·5, 7·0 and 7·5. The specific growth rate and bacteriocin titers were highest at pH 7·0, with values of 0·33 h −1 and 20 000 AU/ml, respectively....
A cDNA encoding human liver UDP-glucuronosyltransferase 2B4, which glucuronidates the bile acid hyodeoxycholic acid, has been expressed in Escherichia coli as a fusion protein with Staphylococcus aureus protein A. The expression of this protein, under the control of the thermo-inducible λ-P R...
Xylitol production by Candida boidinii NRRL Y-17213 was investigated in fed-batch fermentations with xylose (50, 100 g litre −1 ) and a mixture of glucose (25 g litre −1 ) and xylose (25 g litre −1 ). All fermentations were initially batch processes with high levels of aeration and rapid...
The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic...
Bacillus licheniformis 44MB82-A cells were immobilized in alginate and agar gels. The optimal immobilization parameters (gel concentration, initial cell quantity, biomass age, bead size and solidification prolongation) were determined. The immobilization procedure was most effective at a gel...
Previous batch studies have shown that leaf mould is a potentially useful biosorbent for the treatment of wastewaters contaminated with hexavalent chromium. This paper examines its use for continuous adsorption in columns and compares the results with a parallel study using an activated carbon...
β-Glucosidase was adsorbed on chitosan and crosslinked with glutaraldehyde. The enzyme exhibited a considerable affinity to chitosan, giving good immobilization yields (55–85%) while maintaining an optimum level of activity (550–850 U/g). The stability in acid buffer ( T 1 2 >80–100 days )...
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