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The activation of oxygen stress-related enzymes was compared in regenerating and non-regenerating tulip bulb scale explants and regenerating stalk explants. The phospholipid composition of scale explants showed an increase of linolenic acid (1–15%) and a decrease in linoleic acid (70–55%)....
Three bifunctional plant enzymes (named TKC 15, TKC 28-I, and TKC 28-II) with both chitinase and RIP activity were purified from the medium of Trichosanthes kirilowii plant cell cultures. Highly purified preparations of these proteins exhibited endochitolytic activity as well as the specific 28S...
Exposure of the photosynthetic machinery to strong light causes the photoinhibition of the photosystem II complex. The recovery from the photoinhibition in vivo was characterized by monitoring the ratio of variable to maximum fluorescence ( F v / F m ) in detached leaves of broad bean ( Vicia...
Cotyledon explants of Panax ginseng zygotic embryos directly produced somatic embryos on Murashige and Skoog medium without growth regulators. Somatic embryos were formed only near the proximal excised region of cotyledons. Multiple and/or single embryos were formed and the frequency of these...
We isolated a cDNA clone (ATMRK1) with sequence similarity to mammal mixed-lineage kinase homologues and Raf protein kinase homologues in the catalytic domain from Arabidopsis thaliana using the polymerase chain reaction (PCR). The ATMRK1 cDNA encodes a 391-amino acid polypeptide containing all...
The complexity of the regulatory mechanisms that govern amino acid biosynthesis, particularly in multibranched pathways, frequently results in sensitivity to growth inhibition by exogenous amino acids. Usually the inhibition caused by a given amino acid(s) is relieved by another amino acid(s),...
The gai mutation of Arabidopsis confers reduced gibberellin responses, and is inherited as a semidominant trait. Somatic sectors displaying wild-type phenotype were observed in plants which were heterozygous for gai and for a linked Ds element, and which also contained a source of the Ac...
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