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We report the first protocol for callus induction and shoot regeneration for Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva. We used different explants viz., epicotyls, cotyledons and hypocotyls. The medium used was MS basal medium with thidiazuron (0.5 mg l −1 ) and IAA (0.05 mg l...
Several protocols have been proposed for in vitro propagation of papaya, either based on somatic embryogenesis or shoot organogenesis. It is well-known that tissue culture-based approaches are frequently associated with somaclonal variation. Whether on the one hand this phenomenon can preclude...
In order to investigate chromosome elimination in symmetric somatic hybridization between Bupleurum scorzonerifolium and Arabidopsis thaliana , protoplasts were isolated from suspension cultures of both A. thaliana and B. scorzonerifolium parents. Biparental protoplasts were mixed at a rate of...
We investigated the effect of the physical state of the nutrient medium on the induction of somatic embryogenesis on cell cultures derived from coffee ( Coffea arabica L.). Non-embryogenic callus tissues were pulsed initially with 50 μM 6-benzyladenine (BA) for 6, 24 or 48 h in half-strength...
A protocol was developed for regeneration and Agrobacterium -mediated genetic transformation of Lesquerella fendleri . Calli were first induced from hypocotyls and cotyledons on MS plus 0.5 mg l −1 BA, 1 mg l −1 NAA and 1 mg l −1 2,4-D, then co-cultivated for 2–3 days in darkness on MS...
In search for the optimal culture conditions resulting in a high production of healthy plants and low occurrence of hyperhydricity in tissue cultured regenerants of Aloe polyphylla , we investigated the relationship between ammonium ions in the medium, applied cytokinins (CKs) and CK...
Dikegulac, a growth regulator, was shown to stimulate in vitro shoot multiplication of olive cultivars Canino, Frantoio, Moraiolo, but not cultivars Rosciola and Piantone di Moiano. The Rugini Olive Medium including zeatin (4.5 μM) was used supplemented with a range of dikegulac concentrations...
Axenic cultures of Posidonia coriacea and Halophila ovalis were established to investigate the effect of nitrogen addition and the optimum concentration of MS (Murashige and Skoog Physiol Plant 15:473–497, 1962) macronutrients, micronutrients and organics. Chlorophyll content and dissolved...
Abiotic stress, such as extreme temperature, drought, or excessive salinity, is one of the leading causes of crop loss worldwide. Microspore-derived (MD) cell suspension cultures of Brassica napus L. cv. Jet Neuf have been shown to be a useful system for studying the biochemistry of developing...
We developed Glycine max cv MiniMax (PI643148) that has a rapid life cycle, short stature and characteristic simple sequence repeat (SSR) markers that could make it useful for mutant screening, functional genomics, genetic mapping and other studies involving soybeans. We demonstrate that MiniMax...
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