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Phytodecta fornicata Brüggemann is a serious pest of alfalfa ( Medicago sativa L.) that causes significant crop loss in the Balkan peninsula of Europe. We introduced a wound-inducible oryzacystatin II ( OCII ) gene to alfalfa to evaluate its effect on survival of P. fornicata larvae. Feeding...
The aim of this work was to study the effects of colchicine application on chromosome doubling and androgenic response in anther and microspore culture of different bread wheat genotypes. Colchicine was applied during a mannitol stress pretreatment or during the first 48 h of culture at...
The aim of the present work was to study the effect of the developmental stage of the somatic embryos and of the genotype on the genetic transformation of embryogenic lines of European chestnut ( Castanea sativa Mill.) and the cryopreservation of the embryogenic lines that are generated. As an...
A very efficient and rapid regeneration system via multiple shoot formation was developed for Cichorium intybus L. when leaf explants excised from sterile seedlings were cultured on medium supplemented with different concentrations and combinations of various plant growth regulators. In a...
Protoplasts were isolated from cell suspensions derived from cotyledon and hypocotyl Gentiana kurroo (Royle). Cell walls were digested with an enzyme cocktail containing cellulase, macerozyme, driselase, hemicellulase and pectolyase in CPW solution. Protoplast viability ranged from 88 to 96%....
An efficient genotype independent, in vitro regeneration system was developed for nine popular Indian wheat cultivars, three each of Triticum aestivum L. viz., CPAN1676, HD2329 and PBW343, Triticum durum Desf. viz., PDW215, PDW233 and WH896, and Triticum dicoccum Schrank. Schubl. viz., DDK1001,...
Condensation on the lids of Petri dishes, used to culture plant tissues, can often obscure the view of the contents of the dish and interfere with data collection. Under the high humidity conditions that exist in the culture container, a small temperature drop causes water to condense on the...
The majority of potted flowering orchids sold in the United States are Phalaenopsis hybrids. However, demand for other genera will undoubtedly increase as consumers become accustomed to growing orchids in the home. The objective of this study was to compare the germination and development of...
Transgenic herbicide-resistant sweet potato plants ( Ipomoea batatas (L.) Lam.) were produced through Agrobacterium -mediated transformation system. Embryogenic calli derived from shoot apical meristems were infected with Agrobacterium tumefaciens strain EHA105 harboring the pCAMBIA3301 vector...
An efficient Agrobacterium -mediated transformation system, from which transgenic tropical maize plants were directly generated without previous crosses with laboratory or temperate lines, was established. Experimental evaluations were focused on two main issues: (i) establishment of appropriate...
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