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A mass in vitro propagation system for Bacopa monniera (L.) Wettst. (Scrophulariaceae), a medicinally important plant, has been developed. A range of cytokinins have been investigated for multiple shoot induction with node, internode and leaf explants. Of the four cytokinins (6-benzyladenine,...
The effects of NaCl-adaptation and NaCl-stress on in vivo H + extrusion and microsomal vanadate- and bafilomycin-sensitive ATPase and PPase activities were studied in tomato cell suspensions. Acidification of the external medium by 50 mM NaCl-adapted and non-adapted (control) tomato cells was...
Little is known about physiological changes that occur with micropropagated chile ancho pepper ( Capsicum annuum L. cv. San Luis) plantlets during acclimatization. Plantlets were transferred to ex vitro conditions to study selected physiological changes and growth performance during...
Buds and shoot tips of wild bilberry ( Vaccinium myrtillus L.) and lingonberry ( V. vitis-idaea L.) plants were cultured on a modified MS medium containing N 6 -isopentenyladenine (2iP), 9.8–78.4 μM, in order to study the effect of the 2iP-concentration on the initiation of growth. The...
Volatile compounds released from callus and nucellar embryo tissues of ‘Valencia late’ and ‘Washington Navel’ sweet oranges ( Citrus sinensis , L. Osbeck) were collected/concentrated by head space solid phase micro extraction and analysed by gas chromatography-mass spectrometry. Friable,...
Initiation and elongation of lateral buds is stimulated in in vitro -grown shoots of Hancornia speciosa (a tropical fruit tree) by high temperature (35 °C), which is associated with the plant's reduced ability to release ethylene. However, no increase in either lateral shoot elongation or...
Shoot tips excised from glasshouse-raised seedlings and field-grown plants of cashew ( Anacardium occidentale L.) were micrografted by a modified side-grafting procedure using in vitro -raised seedling rootstocks. Factors affecting graft-take were optimised. The micro-scion take obtained was in...
A 90% resource cost reduction in tissue culture of banana was achieved by replacing tissue culture grade sucrose and Gelrite in the medium with locally available commercial sugar and a starch/Gelrite mixture and by using sun light instead of artificial light. The micropropagation of Musa `Grande...
This study reports haploid plantlet regeneration through gynogenesis in Citrus clementina Hort. ex Tan., cv. Nules, induced by in vitro pollination with pollen grains of Oroblanco, a triploid cultivar of grapefruit. It indicates that parthenogenesis induced in vitro by triploid pollen can be an...
An effective protocol has been developed for plant regeneration from cotyledon explants of Swainsona salsula Taubert (Saline swainsona), a medicinal and agronomic shrub. Adventitious shoots were obtained from 83.2% of cotyledon explants from 3-day seedlings cultured on Murashige and Skoog (MS)...
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