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An efficient protocol for the Agrobacterium tumefaciens -mediated transformation of calla lily ( Zantedeschia elliottiana (W. Wats.) Engl. cultivar ‘Florex Gold’) is described. Shoot basal discs were co-cultivated with A . tumefaciens C58C1 carrying a plasmid containing neomycin...
The shoot apical meristem (SAM) produces lateral organs in a regular spacing (phyllotaxy) and at a regular interval (phyllochron) during the vegetative phase. In a Dissociation ( Ds ) insertion rice population, we identified a mutant, compact shoot and leafy head 1 ( csl1 ), which produced...
Fusarium head blight (FHB) is a devastating disease of small grain cereal crops caused by the necrotrophic pathogen Fusarium graminearum and Fusarium culmorum . These fungi produce the trichothecene mycotoxin deoxynivalenol (DON) and its derivatives, which enhance the disease development during...
Zinc finger proteins (ZFPs) play important roles in growth and development in both animals and plants. Recently, some Arabidopsis genes encoding distinct ZFPs have been identified. However, the physiological role of their homologues with putative zinc finger motif remains unclear. In the present...
Vacuolar collapse plays a direct role in the cell death of the interspecific hybrid of Nicotiana gossei Domin × N. tabacum L. which exhibits hybrid lethality at the seedling stage. We have previously reported that cell death in these seedlings began at the base of hypocotyls and spread...
α-Tocopherol is the most biologically active component of vitamin E and is synthesized only by photosynthetic organisms. Two heterotrophic cell lines of sunflower ( Helianthus annuus L.) of differing α-tocopherol biosynthetic capability, three-fold higher in the high synthesizing cell line, HT,...
The objectives of this study were to test the feasibility of introducing barley hva1 gene, a LEA3 member, into perennial grass species using the Agrobacterium -mediated transformation technique and to determine whether heterologous expression of hva1 would alleviate water-deficit injury in grass...
Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a...
Somatic embryos of soybean ( Glycine max (L.) Merrill) have been used to generate transgenic plants by particle bombardment. The induction and proliferation of somatic embryos from immature cotyledons are dependent on the genotype of the cultivar. Whereas somatic embryogenesis and plant...
In this work, Potato virus Y (PVY) resistant potatoes were generated using an environmentally safe construct. For this purpose, a ‘shooter’ mutant Agrobacterium -based transformation system was used. The isopentenyl transferase gene ( ipt ) present on the Ti plasmid of ‘shooter’ strains...
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