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Allocasuarina verticillata is an actinorhizal tree that lives in symbiotic association with a nitrogen fixing actinomycete called Frankia . In the search for promoters that drive strong constitutive expression in this tropical tree, we studied the organ specificity of four different constitutive...
Transgenic sorghum plants expressing a synthetic cry1Ac gene from Bacillus thuringiensis ( Bt ) under the control of a wound-inducible promoter from the maize protease inhibitor gene ( mpiC1 ) were produced via particle bombardment of shoot apices. Plants were regenerated from the transformed...
A reproducible procedure was developed for genetic transformation of grasspea using epicotyl segment co-cultivation with Agrobacterium . Two disarmed Agrobacterium tumefaciens strains, EHA 105 and LBA 4404, both carrying the binary plasmid p35SGUSINT with the neomycin phosphotransferase II ( npt...
A highly efficient, repetitive system of organogenesis was developed in soybean. Seeds of soybean cv. ‘White hilum’ pretreated with TDZ formed multiple bud tissue(s) (MBT) at the cotyledonary nodes. MBT initiation occurred only if the axillary buds were not removed from the cotyledonary node....
The sequence of events in the functional body pattern formation during the somatic embryo development in cowpea suspensions is described under three heads. Early stages of somatic embryogenesis were characterized by both periclinal and anticlinal cell divisions. Differentiation of the protoderm...
Transgenic corn (MON 810), expressing the Bacillus thuringiensis ( Bt ) protein, Cry1Ab, was evaluated under greenhouse conditions for its tolerance to the maize stem borer, Chilo partellus. Bt corn (MON 810) provided effective protection against the stem borer even under a high level of larval...
Vaccines produced by transgenic plants would have the potential to change the traditional means of production and inoculation of vaccines, and to reduce the cost of vaccine production. In the present study, an UreB antigen gene from a new Helicobacter pylori strain ZJC02 was cloned into the...
Transgenic plants with introduced pest-resistant gene offer an efficient alternative insect control. The novel insect-resistant gene combination, chitinase ( chi ) and BmkIT ( Bmk ), containing an insect-specific chitinase gene and a scorpion insect toxin gene was introduced into Brassica napus...
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