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We report the cloning of a glycoside hydrolase family (GHF) 9 gene of rice ( Oryza sativa L. cv. Sasanishiki), OsCel9A , corresponding to the auxin-induced 51 kDa endo-1,4-β-glucanase (EGase). This enzyme reveals a broad substrate specificity with respect to sugar backbones (glucose and xylose)...
Recent completion of the sequencing of the rice genome has revealed that it contains >40% repetitive sequences, most of which are related to inactive transposable elements. During the molecular analysis of the floral organ number1/multiple pistil 2 ( fon1/mp2 ) mutant, we identified an active...
Cytokinins stimulate shoot regeneration in tissue culture but the genes required for this developmental process are not well understood. Here we show that the Arabidopsis gene, ENHANCER OF SHOOT REGENERATION2 ( ESR2 ), encoding an AP2-domain transcriptional factor, functions in the regeneration...
The histochemical and cytochemical localization of water stress-induced H 2 O 2 production in the leaves of ABA-deficient vp5 mutant and wild-type maize ( Zea mays L.) plants were examined, using 3,3-diaminobenzidine and CeCl 3 staining, respectively, and the roles of endogenous ABA in the...
PAMP (pathogen-associated molecular pattern) recognition plays an important role during the innate immune response in both plants and animals. Lipopolysaccharides (LPS) derived from Gram-negative bacteria are representative of typical PAMP molecules and have been reported to induce...
An auxin analog, 2,4-D, stimulates the activity of endo-1,4-β-glucanase (EGase) in rice ( Oryza sativa L.). The auxin-induced activity from three protein fractions was purified to homogeneity from primary root tissues (based on SDS–PAGE and isoelectric focusing after Coomassie brilliant blue...
The wall-yielding properties of cell walls were examined using frozen–thawed and pressed segments (FTPs) obtained from the elongation zones of cucumber hypocotyls with a newly developed programmable creep meter. The rate of wall extension characteristically changed depending on both tension and...
Nodule-specific (nodulin) genes are thought to play crucial roles during establishment of the nitrogen-fixing symbiosis between legume plants and Rhizobium bacteria. On the basis of a gene expression database for early stages of the nodulation process of Lotus japonicus , previously constructed...
We report here the Agrobacterium -mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to...
Transgenic tobacco ( Nicotiana tabacum ) lacking mitochondrial alternative oxidase (AOX) have been compared with wild-type (Wt) tobacco using two different systems, either suspension cell cultures or leaves. In both systems, a lack of AOX was accompanied by an increase in some anti-oxidant...
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