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The roles of actin microfilaments (MFs) in the organization of microtubules (MTs) at the M/G 1 interface were investigated in transgenic tobacco BY-2 cells stably expressing a GFP-tubulin fusion protein, using the MF-disrupting agent, Bistheonellide A (BA). When MFs were disrupted by BA...
The development of root hairs serves as an excellent model to study cell growth using both cytological and genetic approaches. In the past, we have characterized LRX1, an extracellular protein of Arabidopsis consisting of an LRR-domain and a structural extensin domain. LRX1 is specifically...
A large number of proteins in the tonoplast, including pumps, carriers, ion channels and receptors support the various functions of the plant vacuole. To date, few proteins involved in these activities have been identified at the molecular level. In this study, proteomic analysis was used to...
Polyamines play pivotal roles in plant defense to environmental stresses. However, stress tolerance of genetically engineered plants for polyamine biosynthesis has been little examined so far. We cloned spermidine synthase cDNA from Cucurbita ficifolia and the gene was introduced to Arabidopsis...
ABA exogenously applied to the leaves of the whole plants of pear ( Pyrus bretschneideri Redh. cv. Suly grafted on Pyrus betulaefolia Rehd.) significantly increased the betaine concentrations in the leaves when the plants were well watered. The plants subjected to ‘drought plus ABA’ treatment...
A key element in the regulation of mammalian steroid biosynthesis is the 18 kDa peripheral-type benzodiazepine receptor (PBR), which mediates mitochondrial cholesterol import. PBR also possess an affinity to the tetrapyrrole metabolite protoporphyrin. The bacterial homolog to the mammalian PBR,...
A strongly increased ATP/ADP ratio was found during the nocturnal phase I in crassulacean acid metabolism (CAM)-induced Mesembryanthemum crystallinum plants. Conversely, during the daytime phase III in CAM-performing plants the ATP/ADP ratio dropped to a similar level to that of C 3 plants,...
Calreticulin (CRT), a major Ca 2+ -sequestering protein, has been implicated in a variety of cellular functions such as Ca 2+ storage, signaling and chaperone activity within the cytoplasm and endoplasmic reticulum. To investigate the biological role of CRT in rice, 21 partial cDNAs, encoding...
Cytosolic free Ca 2+ serves as an important second messenger participating in signal transduction of various environmental stresses. However, molecular bases for the plasma membrane Ca 2+ influx and its regulation remain largely unknown. We here identified a gene ( OsTPC1 ) encoding a putative...
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