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The vanadate-induced nucleotide trapping technique, which has been conventionally used to characterize mammalian ATP-binding cassette (ABC) proteins, was applied to berberine-producing plant cell cultures, Thalictrum minus and Coptis japonica . One membrane protein at ca. 180 kDa was...
Vesicle traffic plays a central role in eukaryotic transport. The presence of a vesicle transport system inside chloroplasts of spermatophytes raises the question of its phylogenetic origin. To elucidate the evolution of this transport system we analyzed organisms belonging to different lineages...
Canavalia ensiformis (jackbean) seeds contain the proteins urease and canatoxin, a variant form of the jackbean urease. Here we have cloned a cDNA encoding another isoform of urease, called JBURE-II. This cDNA was obtained by RT-PCR using as template total RNA extracted from C. ensiformis...
Cellular and intracellular motile events in plants are susceptible to SH reagents such as N -ethylmaleimide (NEM). It has long been believed that the target of the reagent is myosin. We compared the effect of NEM on the motile and ATPase activities of skeletal muscle myosin with that on plant...
Previous data obtained in different suspension-cultured plant cells have clearly illustrated that N -glycans are absolutely required for transport of glycoproteins to the extracellular compartment, regardless of their oligosaccharide structure (see Lerouge et al. (1998) Plant Mol. Biol . 38: 31...
The superior ability of citrate excretion in a carrot ( Daucus carota L.) mutant cell line, namely IPG (insoluble phosphate grower) (Takita et al. (1999a) Plant Cell Physiol . 40: 489) cells has been characterized in terms of citrate transport at the plasma membrane. IPG cells released about a...
Formononetin (7-hydroxy-4′-methoxyisoflavone, also known as 4′- O -methyldaidzein) is an essential intermediate of ecophysiologically active leguminous isoflavonoids. The biosynthetic pathway to produce 4′-methoxyl of formononetin has been unknown because the methyl transfer from S...
A complete cDNA from Pinus pinaster Aiton, potentially coding for an α-xylosidase able to remove the xylose residue from xyloglucan oligosaccharides, has been cloned. Its sequence was homologous to previously published α-xylosidase genes from Arabidopsis and nasturtium. The protein also showed...
Influxes of glucose, fructose and sucrose were characterised for coat cells of developing seeds of Phaseolus vulgaris L. and Vicia faba L. by monitoring uptake of ( 14 C)sugars into excised seed-coat halves and two different protoplast populations derived from seed coats. Sugar influxes by the...
To understand a physiological role of an abundant 34-kDa periplasmic protein in the denitrifying phototroph Rhodobacter sphaeroides f. sp. denitrificans grown in a medium containing malate as the carbon source, the gene for the protein was isolated. The deduced amino acid sequence of the protein...
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