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Highlights • New strategy to delay compensatory response mechanisms in polyphagous insects. • Pacifastin-like inhibitor peptides inhibit digestive enzymes in desert locusts. • Feeding pacifastin combinations impedes locust larvae growth. • Pacifastin combinations are more effective than...
Highlights ► HWTX-I is a cross channel toxin, in this study, we make sure that the second function (HWTX-I can inhibit the TTX-S VGSCs in rat DRG neurons as well N-Ca 2+ ) is the nature of HWTX-I not from the contamination through testing the synthetic HWTX-I. ► HWTX-I can inhibit the TTX-S...
Highlights ► Biostable insect pyrokinin analogs with Oic show potent oral antifeedant and aphicidal activity. ► A PEG-polymer conjugated pyrokinin analog shows potent oral antifeedant and aphicidal activity. ► Unmodified, native aphid pyrokinins are inactive. ► Aphicidal activity is...
Highlights ► Taenia solium paramyosin protects both the parasite and is vaccine candidate: how? ► Epitopes involved in these functions are unknown. ► Epitope study using synthetic peptide as immunigen and mimotopes from phage library. ► Hypothesis: helical epitopes protect the parasite,...
Highlights ► DdEGFL1 is not a chemoattractant for Dictyostelium discoideum cells. ► DdEGFL1 does not significantly enhance folate-mediated chemotaxis. ► DdEGFL1-enhanced random cell motility requires PLA2 and CaM activity. ► Folate-mediated chemotaxis requires PI3K, PLA2, CaM, and...
Highlights ► We have isolated an adipokinetic peptide from the stick insect Baculum extradentatum . ► The adipokinetic peptide in Baculum is identical to that in Carausius morosus . ► The adipokinetic peptide in Baculum has hypertrehalosemic and cardioacceleratory activity. ► A second...
Highlights ► We set up a cell-based bioassay to screen for peptides with CCK1-receptor activity. ► A plate reader and a confocal microscope were compared to measure the Ca 2+ increase. ► The plate reader assay allowed fast, high throughput screening. ► The microscope identified...
Highlights ► The Kunitz-type multigene superfamily composed of distinct gene families (GS-, RG-, GG-, and GN-) has been found. ► The exon–intron structure of GS-genes has been determined. ► Three full-length and 33 mature cDNAs encoding members of GS-polypeptides have been obtained. ►...
Highlights ► Ecdysteroidogenesis is the only well established function of insect prothoracic glands (PGs). ► Locust prothoracic glands have a well developed rough endoplasmic reticulum: protein secretion? ► Our mass spectrometric analysis did not provide evidence for a role in peptide...
Highlights ► By applying the non invasive imaging mass spectrometry (IMS) on the paraffin-embedded tissue sections (PETS), we were able to identify and map the location of up to 29 physiologically important neuropepetides in the eyestalk, the brain and the thoracic ganglia of Penaeus monodon ,...
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