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(Tyr 0 ,Bpa 4 )bombesin, (YB)BB was synthesized and its biologic activity evaluated using T47D breast cancer cells. ( 125 I-Tyr 0 ,Bpa 4 )BB bound with high affinity ( K d = 5 nM) to T47D cells. Specific ( 125 I-Tyr 0 ,Bpa 4 )BB binding was inhibited with high affinity by BB, BW2258U89, GRP, GRP...
Angiotensin-(1–7) decreased mitogen-activated protein (MAP) kinase (Erks) activation in cultured Mardin–Darby bovine kidney (MDBK) epithelial cells. Also, saturable, high-affinity 125 I-angiotensin-(1–7) binding was detected in MDBK cell membranes. Together, the data suggested the possible...
Peptide YY (PYY)-positive cells are distributed in the mucosa of the ileum, cecum, colon, and rectum of sheep, but not in other layers of these regions. By radioimmunoassay, mucosal content of PYY in the ovine large intestine was much less than that in the rat intestine. The plasma concentration...
Two N-terminally truncated forms of the C-type natriuretic peptide (CNP) were isolated from the venom of habu snake, Trimeresurus flavoviridis , and their structures were determined by EMI-MS spectrometry and amino acid sequencing. Tf -CNP(6–22), the shorter peptide retaining the 17-membered...
Intraplantar injection of the enzymatically stable, N -methylated kyotorphin analog Tyr(NMe)-Arg-OH produced marked and sharp nociceptive flexor responses in a dose-dependent manner. A significant response was observed with this compound at a dose of 0.01 amol (6000 molecules)....
Octopus cardioactive peptides (Ocp-1: Gly- d -Phe-Gly-Asp, and Ocp-3: Gly-Ser-Trp-Asp) were isolated from brain extracts of the octopus, Octopus minor , using the isolated systemic heart as a bioassay. These peptides showed both positive chronotropic and inotropic effects on the heart. The...
This study aimed to characterize the β-endorphin-immunoreactive material (βE-IR) detectable in normal human keratinocytes (NHK). The effects of different culturing conditions and UV-irradiation on production of βE-IR by NHK were assessed by radioimmunoassay and HPLC. All culture systems...
In the migratory locust, the CRF-related diuretic hormone that stimulates fluid secretion by the Malpighian tubules, and the ovary maturing parsin, a neurohormone able to stimulate oogenesis, are produced by the same neuroendocrine cells of the pars intercerebralis in the brain.
To evaluate the release and possible role of N-terminal end of atrial natriuretic factor (ANF) prohormone (proANF-1–30) and C-terminal end of ANF prohormone ( a ANP-1–28) in patients with soft tissue and bone injuries, 20 patients with soft tissue injuries, 18 bone-fractured patients, and 21...
Agouti-related protein (AGRP) is a naturally occurring antagonist of the brain melanocortin receptors (MC3R and MC4R) and is physiologically implicated as participating in feeding behavior and energy homeostasis. The human AGRP decapeptide Yc(CRFFNAFC)Y has been previously reported as binding to...
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