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The effects of a synthetic Met-enkephalin analogue ( d -Ala 2 ,MePhe 4, Met(O) 5 -ol)enkephalin (DAMME) (1 mg/kg, IP) on gastric damage produced by necrotizing agents (0.6 N HCl, ethanol 1 ml/rat, PO) were evaluated, and the correlation between histamine and opioids in stomach was investigated....
We investigated the efficacy and the mechanism of action of EM523L, a nonpeptide motilin agonist (motilide), on the stimulation of gastric emptying and on the release of gut peptides after ingestion of a solid meal in normal controls ( n = 8) and in diabetic patients ( n = 8) with signs of...
To determine whether NPY and 5-HT interact, we studied the effects on hypothalamic NPY and NPY mRNA levels of acute (10 mg/kg) and 7-day administration (10 mg/kg/day) of m CPP (a 5- HT 1B 2C agonist) and flesinoxan (a 5-HT 1A agonist). After both treatments, m CPP reduced food intake and NPY...
Strips of lower esophageal sphincter (LES) from rabbits were tested for their responses to several peptides, and to electrical field stimulation (EFS) under the presence of some peptides. Substance P (SP), motilin, and bombesin induced contraction, and vasoactive intestinal peptide (VIP) induced...
The medial nucleus of the amygdala (Me), bed nucleus of the stria terminalis (BNST), and medial preoptic area (MPOA) regulate copulation in the male hamster. The present study identified neuropeptide Y-immunoreactive (NPY-IR) neurons in the BNST and Me with the greatest concentration in the...
In crustaceans, the endogenous opioid peptides, enkephalins, are known to be concentrated in the thoracic ganglion, although they have been demonstrated in all parts of the nervous system. B max and K d measurements have been obtained for the binding of ligands used to characterize δ- and...
Major complications arising from diabetes mellitus include neuropathic pain and altered peripheral inflammatory responses. Somatostatin (SOM), calcitonin gene-related peptide (CGRP), and substance P (SP) are neuropeptides that modulate pain responses transmitted by primary sensory afferents, the...
We compared the in vitro and in vivo biological activities of PG-KII (pGlu-Pro-Asn-Pro-Asp-Glu-Phe-Val-Gly-Leu-Met-NH 2 ), a new peptide belonging to the tachykinin family, related to kassinin, isolated and sequenced from extracts of the skin of the Australian myobatrachid frog Pseudophryne...
The mechanism by which gastrin-releasing peptide (GRP) increases cytoplasmic calcium (Ca 2+ ) ic was studied in insulin-producing HIT-T15-cells. At zero glucose, GRP (100 n M ) rapidly increased (Ca 2+ ) ic in the presence and absence of extracellular Ca 2+ . The effect was potentiated by...
The effects of captopril, an angiotensin-converting enzyme inhibitor (ACEI), and a selective B 2 kinin receptor antagonist (Icatibant) were examined on the paw edema and tissue contents of bradykinin (BK) and des(Arg 9 )BK following the intraplantar injection of carrageenan in rats. To this end,...
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