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The levels of three proteases in the cerebral cortex of rats following a three week exposure to aluminum, were measured. The activity of apopain (CPP32), an interleukin 1 β converting enzyme (ICE)-like cysteine protease specifically associated with apoptosis, was increased following dosing with...
Monosodium L-glutamate (MSG) causes neuronal lesions in certain brain regions when systemically given to young animals. Also, when glutamate (Glu) builds up in the intersynaptic space, it induces neuroexcitatory and neurocytotoxic effects, events mediated by several Glu receptors. Some of these...
P2Z⧹P2X7 receptor is a particular type of purinoceptor, which is selectively expressedon the surface of immune cells in neuronal and non-neuronal tissues. Despite intensive researchon its involvement in the immune response, the exact mechanism whereby it affects intercellularsignaling is far...
Metallothioneins (MTs) are a family of low molecular weight proteins which in rodents comprise four isoforms (MT-I to -IV). MT-I+II are widely expressed isoforms which are highly inducible by factors such as heavy metals and a number of hormones. The expression of these isoforms in the brain has...
Functional interaction between ionotropic and metabotropic glutamate receptors (iGluR and mGluR respectively) was studied in cerebellar granule cell cultures using quisqualate (QA), the most potent agonist of phosphoinositide hydrolysis coupled mGluR, and N-methyl-D-aspartate (NMDA) or kainate...
A simple and rapid in vitro method for qualitative and quantitative estimation of the Ga-subunits interaction with the third intracellular loop of human D 2s dopamine receptor has been developed. For this purpose, D 2s -CL3 was cloned in pGEX-2T vector and expressed in E. coli BL21 DE3 as a...
Serotonin (5-HT) is a prime candidate for studies of the interaction between the nervous and immune systems, since it is both an important neurotransmitter and released at high concentrations at sites of inflammation. Serotonergic neurotransmission is regulated by the 5-HT transporter (5-HTT),...
Effect of nitric oxide (NO) on striatal acetylcholine (ACh) release induced by N -methyl-D-aspartate (NMDA) was investigated in freely moving rats by means of microdialysis. NMDA caused a significant increase in ACh release in the striatum, which was blocked by the specific NMDA receptor...
The kinetics of L -(14C) arginine ( L -(14C) Arg) uptake and the effects of potential competitors on the uptake were analysed in nonsynaptic mitochondria isolated from rat cerebral hemispheres. Analysis of uptake kinetics revealed a high affinity component with a mean K m =0.08 mM, and V max...
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