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Highlights ► Dimer interfaces of IL-12, IL-27 and IL-35 are characterized by extensive mutagenesis. ► Residues critical to IL-12 and IL-27 dimer formation do not affect IL-35 dimerization. ► IL-35 has distinct subunit pairing criteria relative to IL-12 and IL-27. ► Antibody epitope...
Highlights ► We describe a high, synergistic induction of IDO1 expression by the demethylating drug zebularine and IFN-γ in the human monocytic cell line THP-1. ► The IDO1 promoter is hypermethylated. Exposure of the cells in vitro to IFN-γ alone or in combination with zebularine, results...
Highlights ► HCMV US3 attenuates degradation of MHC class I molecules induced by US11. ► Enhanced ER retention of class I molecules is evident during stable co-expression of US3 and US11. ► Co-infection with US3- and US11-expressing viruses results in robust down-regulation of cell surface...
Highlights ► Chronic portal hypertension in the rat induces a low-grade aortic inflammation. ► Serum cytokines, Corticosterone and ACTH increases in portal hypertensive-rats ► In portal hypertension dyslipidemia is produced. ► Portal hypertensive aorta mRNA expression of oxidative stress...
Highlights ► E2A , PAX5 , CD19 , and IGHD mRNA expressions were reduced in equine CVID patients. ► No mutations in the PAX5 gene were identified in equine CVID patients. ► In equine CVID patients, the pre-pro-B to pro-B cell transition appears impaired.
Highlights ► Deletion of a single cytokine gene IL-6 differentiates naive CD4 + T cells into functional CD4 + Tr1 under Th2 differentiation conditions. ► Deletion of a single cytokine gene IL-10 differentiates naive CD4 + T cells into functional CD4 + Th1 under Th2 differentiation conditions....
Highlights ► LTβR activation on macrophages controls pro-inflammatory cytokine expression. ► LTβR activation by CD4 + T cell derived LTαβ induces TRIM30α expression. ► Intestinal inflammation is controlled and down-regulated by LTβR signaling.
Highlights ► Chaeoglobosin Fex, a marine derived fungal product, suppressed poly(I:C)-induced and TLR3-mediated production of pro-inflammatory mediators in bone marrow-derived DCs (BMDCs). ► The inhibitory activity of Chaeoglobosin Fex was attributed to the down-regulation of phosphorylation...
Highlights ► Three novel scFvs against rabies virus were constructed and expressed in Escherichia coli . ► The stability of the scFv was enhanced by disulfide-stabilization. ► All the scFvs were protective against rabies in postexposure prophylaxis model. ► The in vivo / in vitro...
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