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We have cloned, characterized and sequenced the murine TNF Receptor Associated Factor 1 (TRAF1) gene. Restriction mapping and Southern blotting analysis revealed that the TRAF1 gene comprises 10 exons and 9 intervening introns and spreads over 18 kb of genomic DNA. 5′-RACE analysis of the TRAF1...
A survey of the work with Ig response to allergens carried out previously reveals an allergen-specific response both by IgE and all of IgG subclasses. Response of non-sensitive people is characterized by the appearance of a variety of the IgG subclasses. We have reexamined ragweed and Amb a 1...
Several regions of C9 including three cysteine-rich modules homologous to those in thrombospondin (TS), the low density lipoprotein receptor (LDL), the epidermal growth factors (EDGF), as well as two middle sections of the polypeptide chain were expressed in bacteria. Antibodies derived from...
The heat-shock protein hsp60 is typically found in mitochondria, but, in smaller amounts, also in the cell cytoplasm and associated with the cell membrane. Since heat-shock proteins are known to interact with a variety of molecules and since purified HIV-1 particles were described to contain...
This report describes the correlation between motif-specific hybridization and necleotide sequence as an approach to the identification of individual human V H genes using motif-specific oligonucleotide probes, complementary to specific motifs within individual V H genes. The sensitivity of the...
Dynamic interactions between major histocompatibility complex (MHC) class I heavy chains and β 2 -microglobulin (β 2 m) play a critical role in their stability on the cell surface, and their ability to present peptide antigens to CD8 + T-cells. A cursory review of protein sequence homologies...
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