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The second known case of a skin infection caused by Botryomyces caespitosus is reported. This case has made it possible to describe the characteristics of this fungus in vivo and to establish it as another agent of phaeohyphomycosis.
The 43 kDa glycoprotein (gp43) antigen has been purified by affinity chromatography and Sephacryl S-200 gel filtration from concentrated and dialysed culture supernatant fluids of Paracoccidioides brasiliensis grown in a tomato juice-enriched complex medium (TOM). In the TOM medium, there was...
Cryptococcus neoformans var. neoformans was isolated from domestic dust in 54% of houses occupied or recently occupied by AIDS+ cryptococcosis patients and in 20% of control houses randomly chosen in Bujumbura, Burundi. It was not isolated from wards of similar patients in the University...
Two methods have been used to estimate chitin. In both, the chitin was first converted into chitosan. The insoluble chitosan was then either depolymerized and deaminated with HNO 2 and the product colorimetrically determined with 3-methylbenzo-2-thiazolone hydrazone and Fe 3+ (method A), or...
Nasal and retrobulbar infection caused by the Oomycete Pythium insidiosum is described in a cat. The diagnosis was established on three criteria. The staining of broad, sparsely septate hyphal elements in biopsy tissue using a fluorescein-labelled antiglobulin specific for P. insidiosum ,...
Studies were performed on the mode of action of tolnaftate and resistance to this drug in Microsporum gypseum . Cells grown in the presence of tolnaftate (at the IC 50) showed a reduced content of total phospholipids and sterols whereas there was an increase in total RNA content. Incubation of...
Two Candida albicans sterol mutants deficient in ΔΔ 8,7 -isomerization and 5-desaturation, respectively, were studied in relation to those phenotypes associated with the 14αα-demethylation deficiency, i.e. repression of hypha formation, inability to grow with non-fermentable substrates, and...
A simple and rapid method for extracting specific cell-free antigens (CFA) from the yeast form of Paracoccidioides brasiliensis grown on agar slants was developed. Extracts were analysed mainly by immunodiffusion (ID) tests, for the immunodiagnosis of paracoccidioidomycosis. Extracts were...
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