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The capsule, cell wall, plasma membrane and nucleus, as well as other intracytoplasmic organelles of Cryptococcus neoformans were examined by electron microscopy. The capsule, sometimes thicker than cells, consisted of either coiled, intertwining microfibrils or dots; the dot-type appeared with...
Vinyl plastic tape was used for stripping the horny layer in fungal diseases. Growth of Candida albicans and of dermatophytes was obtained when the tape was subsequently applied to the surface of a Sabouraud's glucose-agar plate.
Among many cases of tinea pedis investigated during the last 20 years, 16 patients were found with ringworm of the feet due to M. canis (7 cases) M. gypseum (5) and M. audouinii (4). Involvement of the toe-nails was observed in 4 cases, 3 by M. canis and 1 by M. audouinii . An animal (cat, dog)...
Antigenic polysaccharide-peptide complexes were extracted from mycelia of Trichophyton mentagrophytes , and purified by gel filtration, DEAE-cellulose and proteolytic digestion. The chemical composition of crude and purified antigens is described. Purified antigens were composed chiefly of...
Serologic studies showed that mice vaccinated intravenously with killed Coccidioides immitis spherules developed higher precipitin titers than mice vaccinated intramuscularly. Nevertheless, the intravenously-vaccinated animals were poorly protected against challenging doses of arthrospores...
A method is described for the isolation of undergraded RNA from yeast-like (Y) and mycelial (M) forms of Paracoccidioides brasiliensis . The method is a combination of various known techniques. Cosedimentation in a sucrose gradient of Y form RNA labelled with 14 C-uracil and M form RNA labelled...
Intramuscular vaccination with killed spherules induced strong immunity to lethal coccidioidal disease in mice. A wide range of dose and small incremental dosage gave uniformly poor protection when administered intravenously. In imuran- or cortisone-treated leukopenic or lymphopenic mice...
A case of pulmonary nocardiosis in a duck is described. Macroscopically, the lesions were nodular, 1–2 mm. in diameter. Histologically there was replacement of normal parenchyma by areas of necrosis and epithelioid and giant cells. The organism was Gram + and PAS + but not acid and alcohol fast.
When incubated at 25° or 37°C for 1–2 days and then placed at 6–9°C for 5–10 days, 29 strains of Candida tropicalis consistently produced terminal bodies similar to chlamydospores. The spores varied in size and shape and many resembled those formed by C. albicans .
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