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The effect of apo very low density lipoprotein (apo VLDL) and apoprotein C-II on the phospholipase A 1 activity associated with lipoprotein lipase (E.C.126.96.36.199) was studied using purified bovine milk lipoprotein lipase. The enzyme degraded 14 C phosphatidylcholine (PC) to 14 C 2-acyl...
The coloring reaction of the thiobarbituric acid test for hydroperoxides was completely inhibited by the addition of EDTA. Therefore, it was necessary to add a metal salt to the reaction mixture to complete the reaction and also to add an antioxidant to prevent autoxidation when unoxidized...
There is considerable evidence that the growing tumor requires a source of unsaturated fatty acids, but the nature of this source and the mechanism of mobilizing the fatty acids from it are obscure. These experiments make use of AKR mice with implanted adipose tisue labeled with 1- 14 C linoleic...
Dolichol from human liver was shown by reverse-phase high pressure liquid chromatography to consist of a series of homologues ranging in length from 17 to 23 isoprene units. The two major components, corresponding to 19 and 20 isoprene units, respectively, were isolated and identified by mass...
The marine sponge Chondrilla nucula contains 34% 30∶3Δ5,9,23 in its total fatty acids. This nonmethylene-interrupted polyunsaturated acid, unknown in other living organisms, occurs mainly as an ester in phosphatidylethanolamine.
Di-(2-ethylhexyl)phthalate (DEHP), a commonly used plasticizer, is now seen as an environmental pollutant. DEHP has been found to inhibit lipid and sterol synthesis in rats and mice. The effects of DEHP on various aspects of lipid metabolism were examined in chickens. White Leghorn laying hens...
Antibodies to pig heart 3-ketoacyl-CoA thiolase inhibited almost completely and in a parallel fashion thiolase and the acetyl-CoA-dependent fatty acid elongation system present in an acetone powder extract of pig heart mitochondria. This finding leads to the conclusion that mitochondrial fatty...
Two unsymmetric polyunsaturated lecithins were allowed to air oxidize and the primary products of autoxidation were isolated and characterized. 1-Palmitic-2-linoleic-phosphatidylcholine undergoes significant oxidation after 16 hr at room temperature under air. A new phospholipid product may be...
After incubation of stationary phase Leishmania donovani with (1- 14 C) octadecanol, about 70% of the precursor was taken up within 3 hr. Wax esters and acyl moieties of glycerolipids contained most of the 14 C-activity from 3 to 6 hr, because octadecanol was partly oxidized to stearate. Ether...
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