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Objective: To determine the effects of single or repeated intramuscular injections of betamethasone, given maternally or directly to the fetus, on chronically catheterized, late-gestation fetal sheep. Methods: Fetal or maternal sheep received either repeated intramuscular injections of...
Objective: To determine the change in expression of the Wilms tumor suppressor gene product, WT1, by progesterone alone in endometrial stromal cell culture and to study its relationship with prolactin, a marker of decidualization. In addition, to examine the change in ratio of WT1 isoforms with...
Objective: This study investigated the response of the human umbilical artery to various agonists in order to determine which agents might be involved in umbilical cord closure. Methods: Umbilical artery rings from 22 cesarean deliveries were suspended in tissue baths containing Krebs-Henseleit...
Objective: Third-trimester human placentas from normal and preeclamptic pregnancies were evaluated for possible changes in gene expression patterns by microarray analysis. Methods: Placentas from four normal pregnancies and four pregnancies complicated by preeclampsia were studied. In a...
There is a growing body of evidence from clinical and epidemiologic studies that in utero exposure to infection plays an important role in the genesis of fetal or neonatal injury leading to cerebral palsy and chronic lung disease. Thus, after chorioamnionitis the incidence of immature neonates...
Objective: Three protease-activated receptor (PAR1, 3, and 4) isoforms have been shown to be responsible for the cellular effects of thrombin; another PAR isoform (PAR2) is responsible for the cellular effects of trypsin. The present studies sought to test the hypothesis that one (or more) of...
Objective: This study was designed to test the hypotheses that disruption of maternal adrenal secretion in late pregnancy requires fetal adaptations in order to maintain fetal blood volume and fetal viability. Methods: Pregnant ewes were adrenalectomized at approximately 112 days, and cortisol...
Background: We tested the hypotheses that (1) maternal betamethasone (ॆM) treatment over the range of clinical doses for prevention of prematurity-related pathologies from day 15 to 21 of rat gestation would produce growth retardation, and (2) the lowest ॆM dose to produce growth retardation...
Objective: Recent reports have indicated that cell-free fetal DNA can be detected in the urine of pregnant women. We attempted to reproduce those data. Methods: Urine samples were collected from 18 normal pregnant women (11 with a male fetus). Urinary DNA was examined by Y-chromosome-specific...
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