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The periplasmic dimethyl sulfoxide reductase (DMSOR) from the photosynthetic purple bacterium Rhodobacter capsulatus functions as the terminal electron acceptor in its respiratory chain. The enzyme catalyzes the reduction of highly oxidized substrates like dimethyl sulfoxide to dimethyl sulfide....
Molecular recognition, site-directed mutagenesis, and molecular modeling are combined to describe hydrogen bonds important for formation and catalysis of the Aspergillus niger glucoamylase-isomaltose complex. This analysis of the energetics of the transition-state complex identified OH-4′,...
The mechanism of selenocysteine insertion into proteins is distinct from all other amino acids in all lines of descent in that it needs specific protein cofactors and a structurally unique tRNA Sec . It is first aminoacylated with serine and further recognized among all other serylated serine...
The operon encoding the periplasmic enzymes dimethyl sulfoxide reductase (DMSOR) and trimethylamine N-oxide reductase (TMAOR) from the purple, non-sulfur, photosynthetic bacterium Rhodobacter capsulatus was isolated, cloned and sequenced, and its chromosomal location determined. It was shown by...
The crystal structure of endo-1,4-β-xylanase I from Aspergillus niger has been solved by molecular replacement and was refined to 2.4 Å resolution. The final R -factor for all data from 6 to 2.4 Å is 17.9%. The A. niger xylanase has a characteristic fold which is unique for family G xylanases...
The crystal structure of the ferredoxin : NADP + reductase (FNR) from the cyanobacterium Anabaena PCC7119 has been determined at 2.6 Å resolution by multiple isomorphous replacement and refined using 15.0 Å to 1.8 Å data, collected at 4°C, to an R -factor of 0.172. The model includes 303...
We have investigated the functional properties of hemoglobin (Hb) valency hybrids, and specifically whether it makes a difference if the oxidized subunits are on the same dimer (asymmetric hybrid) or not. CO recombination kinetics were used to probe the allosteric equilibrium of tetramers with...
Efficient expression of the human immunodeficiency virus (HIV) genome requires the viral-encoded transactivator Tat. Tat interacts with the highly structured trans-activation-response (TAR) RNA that is found at the 5′ end of all viral transcripts, and mediates the formation of transcription...
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