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This report shows that chicken type I and type II interferons (IFNs), like their mammalian counterparts, act synergistically such that a mixture of the two has much greater activity than that expected from the separate contribution of each type. The degree of antiviral synergy was measured by...
Aged monocytes, that is, monocytes purified from the blood of donors â¥65 years of age, when compared with young monocytes, that is, monocytes purified from the blood of young donors 25 years of age, display a decrease in interleukin-6 (IL-6) and tumor necrosis factor (TNF) production after...
The effect of interferon-Î²1b (IFN-Î²) on human cerebral blood flow distribution was examined in five multiple sclerosis patients using functional brain single-photon emission tomography (SPECT). Of nine regions of interest studied, only the basal ganglia exhibited a significant change...
Leukemia inhibitory factor (LIF) is a cytokine that acts on a wide range of cell types in vitro , but knowledge of its physiological role is limited. High levels of LIF protein have been selectively detected in the thymus throughout postnatal development. LIF-deficient mice have shown impaired...
Several Sendai virus (SV) preparations, propagated through eggs from the same viral seed, exhibited significantly different capacities to induce interferon (IFN) in human leukocytes (nHu-IFN-Î±). The amount of induced IFN and the numbers of SV IFN-inducing particles (IFP) per cell were...
We have reported that N -( p -coumaroyl) serotinin (CS) and its derivatives with antioxidative activity are present in safflower seeds. As reactive oxygen species (ROS) are implicated in the signaling of lipopolysaccharide (LPS), we examined whether CS has a suppressive effect on inflammatory...
Controlled-release ethylene-vinyl acetate copolymers (EVAc), which were used previously for the in vivo intracerebral delivery of chemotherapeutics, were evaluated as a possible route of localized intracerebral delivery of interferon (IFN). Natural mouse IFN-Î±/Î² (Mu-IFN-Î±/Î²) was...
We have developed a solid-phase enzyme-linked immunoassay (EIA) for detecting antibodies to interferon-Î±2 (IFN-Î±2) in serum or plasma. In this assay, based on the sandwich principle, the capture antigen, IFN-Î±2, is covalently bound to the wells in 96-well plates. This novel procedure...
Experimental autoimmune encephalomyelitis (EAE) is an inflammatory disease of the central nervous system (CNS). We previously reported upregulation of gene expression for a number of proinflammatory cytokines, interleukin-1Î² (IL-1Î²), IL-2, IL-6, tumor necrosis factor-Î± (TNF-Î±),...
The aim of this study was to investigate interferon-Î³ (IFN-Î³) and interleukin-4 (IL-4) production by peripheral blood mononuclear cells (PBMC) in response to oral challenge in patients with food allergy. Thirty-one patients were compared with 10 healthy subjects. Cell cultures were prepared...
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