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Background We previously demonstrated that a new lentiviral vector derived from nonpathogenic simian immunodeficiency virus (SIVagm) was efficient and safe for long‐lasting retinal gene transfer, and that it provided the significant therapeutic effect of expressing human pigment...
Background To overcome the extracellular barriers in gene delivery and direct gene delivery to target tissues, substrate‐mediated transfection, which sustains the release of naked DNA or vector/DNA complexes, and also supports cell growth, has been developed. Methods In the present study,...
Background Genetic manipulation of the allograft is an attractive approach to prevent the graft against chronic deterioration through stable expression of immunomodulatory or protective genes. However, the best strategy for prevention of chronic allograft deterioration remains unclear. Methods...
Background Purine nucleoside phosphorylase (PNP) deficiency causes the accumulation of toxic purine metabolites and lethal T cell immune defects, which might be corrected by expressing PNP by transplanting bone marrow (BM) cells transduced with lentiviral vectors containing the human PNP gene...
Background The involvement of surface molecules in HIV‐1‐derived lentivirus (LV)‐mediated transduction of airway epithelial cells has not been studied so far. The present study aimed to evaluate the role of glycosaminoglycans (GAGs) in gene transfer mediated by a third generation vesicular...
Background Chemical capsid modification of adenovirus vectors with synthetic polymers has been shown to aid in overcoming typical barriers for adenovirus vector‐mediated gene transfer. Carbohydrate‐based polymers for covalent modification of adenovirus vectors have been largely neglected so...
Background Currently, no treatment is available for food allergy and strict avoidance of the allergenic food remains the only way to manage the allergy. New strategies leading to a safe and efficacious food allergy treatment are required. Modified vaccinia virus Ankara (MVA), which allows high...
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