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Background Luciferase optical imaging provides a novel method to monitor transgene expression in small living animals. As the genetic and immunological heritages of particular animals significantly affect the expression of adenovirus‐delivered transgenes, it is essential to know the expression...
The original article to which this Erratum refers was published in The Journal of Gene Medicine, 6 (10) 2004, 1049–1060.
Background An Erratum has been published for this article in Journal of Gene Medicine 7(6), 2005, 835. A stable packaging cell line facilitates large‐scale lentivirus vector manufacture. However, it has been difficult to produce clinical‐scale HIV‐1‐based lentiviral vectors using a...
Background Delivery of genes to airway mucosa would be a very valuable method for gene therapy and vaccination. However, there have been few reports on suitable gene delivery systems for administration. In this study, we use a cationic emulsion system, which is physically stable and facilitates...
Background The E7 oncoprotein of human papillomavirus type 16 (HPV16) is frequently used as a model tumor‐associated antigen. Its immunogenicity has been substantially enhanced by fusion with several proteins of various origins and functions. Different mechanisms have been responsible for...
Background Following systemic administration, polyplexes must cross the endothelium barrier to deliver genes to the target cells underneath. To design an efficient gene delivery system into lung epithelium, we evaluated capture and transfection efficiencies of DNA complexed with either...
Background The goal of this study was to design improved regulatable lentivirus vector systems. The aim was to design tetracycline (tet)‐regulatable lentivirus vectors based on the Tet‐on system displaying low background expression in the absence of the doxycycline (DOX) inducer and high...
Background Foot‐and‐mouth disease virus (FMDV) causes a severe livestock disease, and the virus is an interesting target for virology and vaccine studies. Materials and methods Here we evaluated comparatively three different viral antigen‐encoding DNA sequences, delivered via two physical...
With the aim of simplifying recombinant‐adeno‐associated virus (rAAV) delivery in muscle, a new femoral intra‐arterial technique was designed and tested in rodents (rats and mice). Two serotypes, several promoters and transgenes (reporter or therapeutic) were tested using this...
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