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10.1002/jcb.240520209.abs We have addressed questions raised by the observation in fetal rats of delayed ossification induced by caffeine at maternal doses above 80 mg/kg body weight per day. The effect of caffeine on endochondral bone development and mineralization has been studied in an...
10.1002/jcb.240520205.abs The emerging picture of cytoarchitecture imposes constraints on the transport and localization of several components of the protein synthetic machinery. The range in which “free” polysomes can diffuse through the cytoplasm may be restricted to about 50 nm due to...
10.1002/jcb.240520206.abs The cytoskeleton of most cells is complex and spatially diverse. The mRNAs for some cytoskeletal proteins are localized, suggesting that synthesis of these proteins may occur at sites appropriate for function or assembly. mRNA concentrations were first observed for...
10.1002/jcb.240520204.abs ABP50 is a polypeptide elongation factor 1α from Dictyostelium that is associated with the actin cytoskeleton. Upon chemotactic stimulation, ABP50 undergoes a dramatic cytoplasmic redistribution into newly formed surface projections and in vitro binds to and bundles...
10.1002/jcb.240520214.abs Atrial natriuretic peptide (ANP) is a fluid‐regulating peptide hormone that promotes vasorelaxation, natriuresis, and diuresis. The mechanisms for the release of ANP and for its clearance from the circulation play important roles in modulating its biological effects....
10.1002/jcb.240520213.abs We investigated the effects of vitamin D3 on the signaling pathways by prostaglandin E2 (PGE2) in osteoblast‐like MC3T3‐E1 cells. The pretreatment with 1,25‐dihydroxyvitamin D3 (1,25‐(OH)2D3), an active form of vitamin D3, significantly inhibited cAMP...
10.1002/jcb.240520211.abs The insulin‐like growth factors (IGFs) have been implicated in the growth regulation of human breast cancer. Since the IGFs are associated with specific binding proteins (IGFBPs) which may modulate receptor/ligand interactions, production of IGFBPs by breast cancer...
10.1002/jcb.240520207.abs ADP‐ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with 32P‐NAD; then the nucleolar proteins were analyzed by 1‐dimensional and 2‐dimensional polyacrylamide gel electrophoresis (PAGE)...
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