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Microorganisms were among the first tools used for the discovery of biologically active compounds. Their utility reached a zenith during the era of antibiotic development in the 1950s and 1960s, then declined. Subsequently, a substantial role for microorganisms in the pharmaceutical industry...
Membranes prepared from stable transfected cells expressing the human gene encoding a functional 5-hydroxytryptaminelE (5HT1E) receptor were used to investigate high-affinity 3 Hserotonin ( 3 H5-HT) binding with scintillation proximity assay (SPA) technology. In this nonseparation format,...
Activation of p34 cdc2 requires a complex series of protein/protein interactions and specific phosphorylation and de-phosphorylation events to occur. The cellular role of p34 cdc2 kinase lies in the regulation of eukaryotic cell entry into mitosis, in particular into the G 2 /M transition. This...
We have developed a scintillation proximity assay (SPA) that allows the Ca 2+ /calmodulin (CaM)-dependent Serine/threoine (Ser/Thr) phosphoprotein phosphatase 2B (calcineurin) activity to be analyzed. A 33 P labeled and biotinylated peptide containing a partial sequence of the regulatory subunit...
Binding of a protein at a ligand-modified ion channel can lead to closure or distortion of the channel, resulting in a digital on/off response of the ion flux. This principle could be applied as a strategy for sensor design using artificial membrane ligands or receptors. Important details of...
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