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Macrophage migration inhibitory factor (MIF) has been found to be widely expressed in many cell types throughout the body and appears to play several physiologic roles. We sought to determine the expression and cellular distribution of MIF in human fetal (HFA) and adult (HAA) adrenals. A single...
This study demonstrates the involvement of phosphotyrosine phosphatases on the activity and regulation of GSH ATP‐dependent transport system that we have previously identified in NIH3T3 fibroblasts. This is shown by the fact that increases of the initial rate of GSH uptake were measured in...
Nearly one‐third of Krupple‐type C2H2 zinc finger proteins have a krupple‐associated box (KRAB) domain, which may act as a transcriptional repressor. ZNF268, which was novelty isolated from early human embryo, is a typical krupple‐type C2H2 zinc finger protein with a conserved KRAB...
The 17β‐estradiol (E2) action mechanism for inducing target gene expression can be attributed to both the direct binding of its receptor (ER) to specific sequences, the estrogen response element (ERE), and to the interaction between ER and other DNA‐binding transcription factors. At the...
Growth factor binding events to receptor tyrosine kinases result in activation of phosphatidylinositol 3‐kinase (PI3K), and activated PI3K generates the membrane‐bound second messengers phosphatidylinositol 3,4‐diphosphate (PI(3,4)P2) and PI(3,4,5)P3, which mediate membrane translocation of...
In this study we used a newly isolated osmo‐, salt‐, and alkali‐tolerant Yarrowia lipolytica yeast strain, with a unique capacity to grow over a wide pH range (3.5‐10.5). A procedure was elaborated to follow phosphate accumulation by Y. lipolytica cells grown at different pH values. In...
A new PCR based method was developed to detect deleted mitochondrial DNA (mtDNA). Peripheral blood cell DNA was obtained from a victim who was accidently exposed to a 60Co radiation source in 1990. Using the DNA as template, first PCR was performed to generate multiple products including true...
The pathogenetic mechanism of the most extensively investigated A3243G mutated tRNALeu (UUR) gene, which causes the MELAS encephalomyopathy, maternally inherited diabetes, or chronic progressive external ophlthalmoplegia, is still unresolved, despite the numerous investigations on the topic....
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