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Inorganic phosphate (Pi) is accumulated by Yarrowia lipolytica cells grown at acidic pH conditions by two kinetically discrete H+/Pi‐ cotransport systems with apparent Km values for Pi of 12‐18 muM and 2‐3 mM Pi at pH 5.5, respectively. One of these is derepressible and operates at low...
A portion of the mouse complement factor I (mCFI) gene encoding for the mCFI light chain was cloned from a mouse 129/SVJ1 bacterial artificial chromosome library. It contains five exons and four introns. The intron sizes are remarkably different from the human homolog. Several polymorphisms were...
The synthesis and applications of carbon nanotubes, a new form of crystalline carbon, are reviewed. In particular, the results obtained in the authors' laboratory are summarized, including the preparation of highly crystalline multiwalled carbon nanotubes; also discussed are the valence band and...
Throughout evolution, both prokaryotic and eukaryotic cells have developed a variety of biochemical mechanisms to define the direction and proximity of extracellular stimuli. This process is essential for the cell to reply properly to the environmental cues that determine cell migration,...
A vanadium‐binding protein was isolated from the culture medium of the vanadium‐reducing bacterium Pseudomonas isachenkovii by utilizing vanadate as the terminal electron acceptor upon anaerobic respiration. The protein was associated with vanadium at a molar ratio of approximately 1:20. It...
In vitro and in vivo studies were carried out to assess the delayed neurotoxicity potential of dichlorvos. In vitro, dichlorvos caused a concentration and time‐dependent decrease in the activity of neuropathy target esterase (NTE). The Ki of dichlorvos for NTE was calculated to be 1.28 × 103...
Shamouti phosphofructokinase (PFP) activation depends on the presence of fructose 2,6‐bisphosphate (Fru‐2,6‐P2) in the glycolytic reaction. The effect of activation by Fru‐2,6‐P2 differs considerably, however, according to the buffer (pH 8.0) in which the reaction is performed: Ka =...
A fully active analog of human parathyroid hormone (hPTH) has been produced by recombinant expression in Escherichia coli. Initially, a nucleotide sequence encoding hPTH(1‐34)‐Asp‐Pro was ligated to a proinsulin gene in the plasmid pUC8, for the eventual expression of a fusion protein of...
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