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By means of the quartz crystal microbalance (QCM), a convenient method was developed to determine the degree of orientation of purple membrane (PM) sheets on the air/water interface. Langmuir‐Blodgett films from both wild‐type and SH‐mutant PM (bR D36C) were vertically deposited on the...
A new trypsin inhibitor (CPTI) has been isolated from Crotalaria paulina seeds. Purification of the inhibitor was carried out by gel filtration, ion‐exchange chromatography, and subsequent reversed‐phase HPLC. The presence of a single polypeptide chain, with a molecular mass of 20 kDa and...
trans‐4‐Phenyl‐3‐buten‐2‐one (PBO), a flavoring additive, was transformed to the carbonyl‐reduced product, trans‐4‐phenyl‐3‐buten‐2‐ol (PBOL) by rat liver microsomes, but not by liver cytosol, in the presence of NADH or NADPH. PBOL formed was identified by comparison...
Semaphorins, the plexin family of semaphorin receptors, and scatter factor receptors share evolutionarily conserved protein modules, such as the semaphorin domain and Met Related Sequences (MRS). All these proteins also have in common a role in mediating cell guidance cues. During development,...
As a step toward analyzing the serine biosynthetic pathway in mammals, we have studied the properties of phosphoserine aminotransferase, the second step‐catalyzing enzyme. The Km values for 3‐phosphohydroxypyruvate and L‐phosphoserine are 5 and 35 mu M, respectively, and those for glutamate...
A murine phage antibody library containing 1.7 108 independent clones was constructed and then screened by affinity panning of anti‐ricin antibody fragments. First, mRNA was purified from the total RNA extracted from fresh spleens of nonimmunized mice of Kunming, and then the total antibody...
Affinity‐purified major histocompatability complex (MHC) class II molecules are known to bind antigenic peptide in vitro. This peptide‐bound MHC class II is known to undergo a change in structure upon stable binding of antigenic peptide. Previous results from our, and other laboratories, have...
The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK‐2123), as well as the rates of lucigenindependent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the...
Thiol‐disulfides cause a time‐ and a concentration‐dependent inactivation of the low‐Mr phosphotyrosine protein phosphatase (PTP). We demonstrated that six of eight enzyme cysteines have similar reactivity against 5,5‐dithiobis(nitrobenzoic acid): Their thiolation is accompanied by...
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