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Three tyrosinase isozymes were purified to electrophoretic homogeneity from pine needles. The molecular weight of the three isozymes (P1, P2 and P3) were approximately 65000, 50000 and 45000, and the pI values were 6.2, 5.9 and 5.3, respectively. The three isozymes have a number of common...
The effects of estradiol and progesterone on the expression of cytochrome P4501A1 were investigated in Hepa lclc7 cells. Both steroids, at 10 μM concentration, increased P4501Al‐mediated 7‐ethoxyresorufin O‐deethyalase activity and amounts of its immunoreactive protein and CYP1AI mRNA Gel...
In order to determine the rote of glutathione reductase (GR) in protection against AI‐phtalocyanine tetrasulfonate‐sensitized human erythrocyte photolysis, we have studied the effects of antitumour alkylating agents that inactivate GR, on photohemolysis rate. The rates of inactivation of...
Poikilocytosis of red blood cells (RBCs) was observed to be associated with anemia in rats given subcutaneous injections of cadmium (Cd). Phase‐contrast light and scanning electron microscopic examinations revealed that acanthocytes appeared in the early stages of administration, and that the...
Deinococcus radiodurans is characterized by its extreme resistance to ionizing radiation. Although Mn+2 was taken up by the cells at a high speed, the absorption of Mn+2 by the cells did not affect their survival rate when irradiated by UV light. However, the Mn‐induced‐cell‐division...
A role of Ca2+‐ and calmodulin‐dependent protein kinase II (CaMK II) in lung surfactant secretion was evaluated using KN‐62, a specific inhibitor of CaMK II. KN‐62 caused a dose‐dependent inhibition of Ca2+ iononphore A23187‐stimulated phosphatidylcholine (PC) secretion from cultured...
Estrogen receptor α was overexpressed in COS‐7 cells by transformation of an expression vector with the full length open reading frame of the receptor α. The nuclear estradiol‐receptor complex and the soluble receptor from the COS‐7 cells were cross‐linked with an estrogen response...
The effects of repetitive mild heat shock (30 min, 41° C) on growth and various cellular and biochemical characteristics of human skin fibroblasts undergoing ageing in vitro were analysed. Human skin ceils not only tolerated more than 30 repeated heat shocks throughout their replicative...
We demonstrated activation of transcription factor AP‐1 in rat nephrotoxic serum (NTS)‐induced glomerulonephritis in a previous report. Here, we evaluate c‐Jun N‐terminal kinases (JNKs) activity to clarify the molecular mechanisms of AP‐1 activation in nephritic glomeruli. Increased...
Rat pancreatic islets were found to display a much lower content of immunoreactive CD38 and a much lower ADP‐ribosyl cyclase activity than rat spleen or brain. Cyclic ADP‐ribose was also measured by a radioimmunological procedure in rat pancreatic islets. In fed rats, the cyclic ADP‐ribose...
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