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One mutant proinsulin gene was constructed through PCR with the code of A19Tyr changed into that of Phe. The mutant proinsulin, (A19Phe)‐lysproinsulin (F19KPI) was expressed in E. coli and purified. After trypsin and carboxypeptidase B cleavage and ResourceTM Q separation, (A19Phe)‐human...
A gene encoding cellulase‐free xylanase was cloned from the thermophilic bacterium Bacillus sp. KK‐1 into Escherichia coli and the gene product was purified from the recombinant E. coli. This xylanase gene, designated xylY, was composed of 1,302 base pairs and encoded a polypeptide of 434...
The RT‐PCR technique was adopted to amplify variable region of VP2 gene of infectious bursal disease virus using three different sets of primers. These primers could generate products of 643, 474 and 552 bp sizes. The authenticity of the amplicons was confirmed by their size in agarose gel,...
β‐N‐Acetylhexosaminidase was purified from the extract of cabbage by sequential steps of ammonium sulfate fractionation, chromatofocusing, DEAE‐Sepharose CL‐6B ion exchange chromatography and Sephacryl S‐200 HR gel filtration. By these steps, the purity of the enzyme increased by 256...
Adriamycin has a wide spectrum of antitumor activity with dose related cardiotoxicity as a major side effect. The objective of this study was to investigate the influence of captopril, a sulphydryl containing angiotensin converting enzyme inhibitor, on the cardio‐and hematotoxicity of...
Cytochrome P450 2B2 and 2B1 are induced in mammalian liver in response to barbiturates such as phenobarbital. The induction of the proteins is mediated at the transcriptional level. Owing to the lack of a suitable phenobarbital‐responsive cell line to investigate the molecular mechanism...
The effects of La3+ on the structure and function of human erythrocyte membranes were investigated by fluorescence polarization, spin‐labeled electron spin resonance (ESR) and differential scanning calorimetry (DSC). The results showed that increasing concentrations of La3+ inhibited...
Two chymotrypsins were purified from the hepatopancreas of grass carp (Ctenopharyngodon idellus) by chromatographies on phenyl‐Sepharose and Q‐Sepharose. The molecular weights of chymotrypsins I and II were 28 and 27 kDa, respectively. The two chymotrypsins showed similar susceptibility to...
Previous results showed that the synthetic compound amidinopiperidine‐4‐carboxylic acid 4‐tert‐butylphenyl ester (APCA‐OPhBut), a trypsin inhibitor, could specifically inhibit the activity of proteinase In and lead to growth arrest of Hela cells in early S phase. In this study,...
The matrix metalloproteinases appear to play a key role in mammary tissue remodeling during involution. By immunoprecipitation and immunoblot using antibodies against 60K gelatinase which appears during involution, a 90K polypeptide has been identified as its inactive proenzyme in the early...
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