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To further determine the role of androgens in the seminal vesicle physiology, we previously undertook cDNA cloning of androgen‐responsive mRNAs from rat seminal vesicles, and isolated several cDNA clones. During the process of characterization, we have isolated a distinct cDNA clone designated...
The fluorescence probe, 8‐anilinonaphthalene‐1 sulfonate (ANS), is used to study the thermal denaturation of adenylate kinase (AK). Native AK has one ANS binding site. With increasing temperature, the ANS binding fluorescence intensity increases, showing that the enzyme undergoes a thermally...
p21, a protein kinase inhibitor, was known to play a major role in the induction of G1 arrest in response to DNA damaging agent, but its role in G2/M transition was poorly understood. In this study, we investigated the involvement of p21 in mitomycin C‐induced G2 arrest in LP1‐1 cells....
Aquaporin (AQP) is the water channel protein associated with water‐permeability, in which AQP1, AQP3, AQP4, and AQP5, are expressed in respiratory organs. Because water filling the fetal lung has to be absorbed after birth, aquaporins are postulated to contribute to this water reabsorption. To...
We prepared three different water‐soluble chitosan derivatives by the acylation of chitosan with corresponding acid anhydrides. The extent of acylation was determined by using the electric conductivity method. All of the chitosan derivatives were soluble enough to examine the respective...
The purpose of this study was to investigate the regulation of V1a receptor gene expression in mouse early proximal tubule (S1) cell line (NF‐5). NF‐5 cells showed arginine vasopressin (AVP)‐induced increase in cytosolic free calcium concentration mediated by V1a receptor. Polymerase chain...
The Authors describe the purification of swine serum ACE to molecular homogeneity with high recovery of activity (40 %) in a few stepsl. The purification procedure consists of affinity chromatography, using commercial activated resin (epoxy‐activated sepharose 6B) and two steps of anion...
Green tea catechins were given to healthy volunteers under controlled diet for 4 consecutive weeks. Plasma concentrations of ascorbate, urate, α‐tocopherol, β‐carotene and blood levels of total glutathione were measured before, after the supplementation and the washout period. RBC membranes...
The effects of interaction of ethanol, l‐butanol or t‐butanol peroxyl radicals with oxyhemoglobin (HbO2) were estimated using absorption spectroscopy in the visible range and tryptophan fluorescence. Peroxyl radicals were generated by gamma‐radiation. Alcohol peroxyl radicals showed the...
By genomic Southern hybridization using degenerate oligo probes corresponding to the conserved zinc finger of the GATA family, four and three new GATA‐factor genes were detected in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. Through degenerate PCR and Southern...
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