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The effect of cations on the kinetics of hemolysis caused by organotin compounds was studied. The ions used in the investigation diminish or totally inhibit hemolysis of red cells induced by organotin compounds. The degree of inhibition depends both on the kind of ion and the compounds that...
The superfamily of adenylate forming enzymes including peptide synthetases, acyl‐CoA synthetases and insect luciferases is readily identified by the signature sequence SGTTGXPKG. This sequence including an invariant lysyl residue is located in a disordered loop region and was predicted to be of...
Mutations of p53 tumor suppressor gene are the most common genetic alterations in a variety of human carcinomas. The sites of p53 mutations, however, vary in different cancers. The present study was designed to characterize p53 mutations in 40 primary human renal cancer specimens using...
Quantitative trait loci (QTLs) affecting body weight were investigated in the backcross population derived from diabetic BB/OK and spontaneously hypertensive rat (SHR). The F1 hybrids were backcrossed onto BB/OK rats, and QTL analysis was performed with the resulting backcross population on...
For the rapid identification of noble genes in a specific tissue by computer analysis from the cDNA sequences determined by single‐pass cDNA sequencing, clone redundancy was one of the major obstacles. To facilitate the efficiency in identification of noble genes, it was necessary to reduce the...
To study the sequential steps in the processing pathway of the chloroplast monocistronic intronless tRNA precursors, we examined cucumber chloroplast tRNALLeu(CAA) processing in a cucumber or pea chloroplast soluble extract. The tRNALeu(CAA) precursor synthesized from SP6 RNA...
Polyacrylamide gel electrophoretic behavior of boar transition protein 1, TP1, under dissociating and non‐dissociating buffer conditions, and titration of fluorescently labeled TP1 with increasing amounts of TP1 showed that TP1 formed a dimer without intermolecular disulfide bond. TP1 dimer...
We have characterized a DNA binding protein (DBNP‐B) from the thermoacidophilic archaeon Sulfolobus acidocaldarius with respect to its interaction with single and double stranded DNA. The protein in solution exists predominantly as dimer as indicated by cross linking studies. Binding of...
It is well established that polyethylene glycol (PEG) shifts the equilibrium in oligomeric protein systems to form higher molecular weight associates. We used this effect of PEG to evaluate a modification of functional properties of LDH from pig skeletal muscles. PEG decreases the rate of...
Free and membrane‐associated fractions of protein kinase C (PKC) from rainbow trout (Onchorynchus mykiss) brain tissue were separated by hydroxylapatite chromatography and characterized kinetically. In both resting fish and in fish swum to exhaustion, approximately 40% of the total PKC activity...
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