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Large quantities of recombinant acid α‐glucosidase are needed for in viva experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A affinity...
In this study we investigated the contribution of pH, phosphate anions and salt concentration to the catalytic and structural thermostability of the carboxypeptidase A (CPA). The concentration of 75‐100 mM phosphate as well as neutral pH values were found to be optimal for stabilizing CPA at...
Beta‐lactamases have been reported to be largely responsible for beta‐lactam resistance in Mycobacteria. We report the characterization of a cell‐associated beta‐lactamase from Mycobacterium smegmatis. The enzyme hydrolyzed the “beta‐lactamase‐stable” oximinocephalosporins....
A cDNA clone encoding human NADH:ubiquinone oxidoreductase (complex I of mitochondrial respiratory chain) MLRQ subunit was isolated from human fetal liver cDNA library. The clone contained an open reading frame of 246 bp which predicted a protein comprising 81 amino acids with a calculated...
The anti‐morphine peptide from canine brain which is homologous to α subunit of hemoglobin of canine, has been isolated and characterized. The canine brain was homogenized and extracted in 0.1 M acetic acid. The supernatant was collected, and purified by chromatography of Sephadex G‐50 and...
The four substrate‐binding sites in porcine liver pyruvate kinase have been labeled with the photoaffinity reagent 8‐azido‐2′‐O‐dansyl‐(α‐32P)ATP (AD‐ATP) under different experimental conditions. In the dark, the native pyruvate kinase was reversibly and competitively inhibited...
The effects of high hydrostatic pressure on the protein synthesis activity of rat liver ribosome and the reconstructed cell‐free translation system were studied. The results indicated that the activity of the ribosome decreased with the increase in applied pressure and the activity was totally...
In Populus deltoides, a deciduous tree, the development on new leaves starts in the month of March, the leaves reach maturity by October and fall by December. Changes in the composition and function of the photosynthetic apparatus were analysed during autumnal senescence. With the progress of...
Amiloride, a diuretic and antihypertensive drug, inhibits Na+ transporting systems, diamine oxidases and the human urinary plasminogen activator. Present results indicate that amiloride is a competitive inhibitor of mouse brain nitric oxide synthase (NOS; Ki= 4.5×10‐4 M, at pH 7.5 and...
cFBP is studied for its affinity to Mg++ and Fru‐1,6‐P2. The affinity for Mg++ is not very high with a Km of 0.24±0.01 mM. High concentrations of Mg++ are inhibitory. The saturation curve for Fru‐1,6‐P2 is hyperbolic with a Km of 0.54±0.014 μM. The presence of citrate (10 mM) induces a...
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