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Bio‐Normalizer, a natural health food supplement prepared from Carica papaya and some other medicinal plants was investigated to determine its effects on cellular nitric oxide (nitrogen monoxide, NO) production and inducible nitric oxide synthase (iNOS) expression. Bio‐Normalizer upregulated...
Non‐receptor tyrosine kinase Chk has been implicated in hematopoietic development. To study the function of Chk in vivo, we have generated chk‐deficient mice using gene targeting. Overall development of mice homozygous for this mutation was apparently normal. Blood counts, FACS analysis of...
Using photochemiluminescence, the interaction between carnosine and superoxide anion was measured directly. Carnosine at physiological concentrations decreased the amplitude of luminol chemiluminescence like superoxide dismutase (SOD) did, and prolonged the lag‐period of the chemiluminescence...
o‐phthalaldehyde inactivates homodimeric, NADP+ dependent, 6‐phosphogluconate dehydrogenase from sheep liver, upon formation of a single isoindole derivative per enzyme subunit. This indicates that the thiol group of a cysteine residue or the ϵ‐amino group of a lysine residue located...
The binding of bromocresol green to bovine serum albumin at micromolar concentrations leads to quenching of protein fluorescence. This property has been used here to study interaction of bromocresol green with bovine serum albumin as a function of pH and ionic strength. The transformation of...
Phospholipases A2 containing Lys‐49 have been reported to be extremely weak or inactive as enzyme. We have recently shown that basic proteins I and II (BP‐I and BP‐II), Lys‐49‐PLA2s isolated from the venom of Trimeresurus flavoviridis (Habu snake), are potent to hydrolyze the...
Nitric oxide (NO) is a powerful vascular and neural regulator. One of the breakdown products of nitric oxide is nitrite which converts to nitrous acid, a reagent routinely used for the degradation of heparin and heparan sulfate. We have recently shown that nitric oxide gas degrades heparin and...
Mutant human tumor necrosis factor alpha(hTNF α ) genes have been constructed by in vitro mutagenesis and expressed in Escherichia coli. A deletion involving Gly68 to His73 in hTNF α remarkably decreased the solubility and biological activity of hTNF α. From the above and results of a...
Preferential codon usage within synonymous groups (codon bias), is hypothesised to be a consequence of either mutational pressure or translational selection. This paper examines PLP and DM‐20, two transcripts differentially spliced from the plp gene, using a variety of previously described...
There are no reports concerning glutathione‐S‐conjugates transport in prokaryotic cells, although many studies have been performed in eukaryotic systems. This study demonstrates that glutathione S‐conjugates (2,4‐dinitrophenyl‐S‐glutathione and bimane‐S‐glutathione) are...
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