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Silver‐stainable proteins (SSPs) are aspartic acid‐rich nuclear proteins which are silver stained under very specific conditions. Using a degenerate oligodeoxynucleotide probe which codes for acidic amino acid residues, a cDNA for a new SSP, referred to as SSP29, has been isolated. The...
In this study, using zymogram analysis two proteolytic activities were identified in the mouse sarcoma 180 (S‐180) cells that were activated by trypsin treatment and inhibited by both BBI and ACTI. These enzymes, with molecular weights of 46 kDa (dominant band) and 62 kDa (minor band), were...
Gene transfer into hematopoietic progenitor cells is increased when these cells are adherent to the carboxy‐terminal chymotryptic fragment of human plasma fibronectin (FN30/35) containing the heparin binding domain (HBD) and the alternatively spliced type three connecting segment (IIICS)...
Evidence showing that cytochrome P450‐mediated reduction of brucine N‐oxide to brucine by rat liver microsomes proceeds nonenzymatically in the presence of both a reduced pyridine nucleotide and FAD is presented. The microsomal N‐oxide reduction appears to proceed in two steps: The first...
We recently isolated stable transfectants expressing human phospholipid hydroperoxide glutathione peroxidase (PHGPx) from the cells of guinea pig cell line 104C1 (Biochem. Biophys. Res. Commun. 219, 486‐491, 1996). Among them, one transfectant, designated 104C1/O4C, expressed high glutathione...
The aim of this study was to demonstrate that modification of the cellular redox‐equilibrium occurs as a consequence of antioxidant nutrients intake (carotenoids, vitamine E and vitamine C) and that these nutrients play a role in the pigmentation of the skin without any UV exposure. We...
In order to demonstrate that the mitochondrial electron transport system may be a target for antimalarial drug design in the human malarial parasite Plasmodium falciparum, ubiquinol‐cytochrome c reductase and cytochrome c oxidase were purified from mitochondria of the parasite cultivated in...
Manganese superoxide dismutase (Mn‐SOD) in the mycelium of Ganoderma microsporum was purified to homogeneity by heat treatment at 70°C, ammonium sulfate fractionation, DEAE‐52 anion‐exchange chromatography, and Sephacryl SH‐200 chromatography. The molecular mass of its native form was...
Oxidative inactivation of AMP deaminase and its protection were analyzed under the in situ conditions of yeast cells. AMP deaminase was readily inactivated by an exposure to hydrogen peroxide plus copper in permeabilized yeast cells. Addition of ascorbic acid further enhanced the inactivation of...
The single chain ribosome‐inactivating protein Saporin‐S6 (SO‐6) induces the fusion of acid phospholipid vesicles. The extent of fusion was measured by resonance energy transfer assay between the N‐(7‐nitro‐2‐1,3‐benzoxadiazol‐4‐yl)‐dimyristoylphosphatidyl lithanolamine...
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