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Acid cysteine proteinase in the eggs of the silkmoth, Bombyx mori, exists as an inactive proenzyme. This 47‐kDa pro‐BCP1 zymogen molecule can be processed in vitro into an enzymatically active 39‐kDa BCP molecule (11, 12). In this current study, the maximum rate of processing in vitro was...
The processes of thrombin‐induced gelation of the fibrinogen may be influenced by a variety of factors including phosphorylational state of the fibrinogen. Casein kinase II (CKII) has been shown to phosphorylate fibrinogen in vitro and accelerate gelation of the fibrinogen. In this work, we...
In this work we focused our attention on some catalytic site requirements for human placenta cytidine deaminase (CDA). The effect of pH on substrate binding and catalysis was studied between pH 3.0 and pH 11.0. The results could be discussed postulating the presence of two classes of ionizable...
Three different lipophilic nitroxyl‐probes having capability to pass the blood‐brain barrier were, for the ftrst time, synthesized to estimate free radical reactions in brain of living animals. Two of the three were designed to be hydrolyzed by esterase and remain in cell. All 3 probes had...
During chick limb development the expression of Hoxa‐11 is specifically associated with the prospective zeugopod (radius+ulna) region. A series of micromass cultures were performed using mesenchyme from whole stage 18/19 or 20/21 limbs or mesenchyme from the tips of stage 22/23, 23/24 or 25...
An A to G point mutation at nucleotide position 7445 in the mitochondrial DNA genome is associated with maternally‐inherited sensorineural deafness in two separate human pedigrees. To determine whether this point mutation (tRNASer(UCN)/COI A7445G) led to sensorineural deafness by affecting...
1‐(5‐Isoquinolinesulfonyl)‐2‐methylpiperazine hydrochloride (H‐7), an inhibitor of protein kinases, has been shown to inhibit the thymocyte apoptosis induced by various apoptogenic agents. In the present study, when mouse thymocytes were pretreated with H‐7, washed, and cultured for...
Plasminogen activator inhibitor‐1 (PAI‐1) cDNA was expressed in Escherichia coli (E. coli) with high efficiency using a heat‐inducible vector. About 100 mg of recombinant PAI‐1 (rPAI‐1) could be obtained from 1 liter of bacteria culture, rPAI‐1 in inclusion bodies was purified by pI...
We investigated the effect of a single bout of intensive exercise on the excretion of 8‐hydroxy‐deoxyguanosine in the 24 h urine from healthy non‐smokers. We studied three exercise tests in Experiment 1; which consisted of incremental exercise to exhaustion on a treadmill in eleven male...
A gel penetration technique, that measures the dilution undergone by protein equilibration on a short tightly packed gel column, has been employed to determine the molecular masses of aldolase (160 kDa), glyceraldehyde‐3‐phosphate dehydrogenase (GPDH; 145 kDa) in the absence and presence of...
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