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“βCATECHIN”, a preparation of natural vitamins and phytosubstances, has been designed as an “universal antioxidant” drink. Having previously demonstrated the scavenging action of a variety of free radicals by “βCATECHIN”, we now report the effect of its long renal administration on...
Seeds of Dolichos lablab var. typicus (Indian lablab beans contain a glucose/mannose specific lectin that was affinity purified on Sepharose mannose columns in our laboratory. The unbound fraction from this matrix showed α‐mannosidase activity. In the present study this has been purified to...
The results in this paper indicate that mitochondrial permeability transition is activated by dysfunction of the respiratory chain caused by anaerobiosis, exhaustion of the oxidative substrate, and antimycin A, in the presence of 100 μM Ca2+. Membrane damage coincides with the collapse of the...
Retinol binding protein prepared from human urine was fractionated by chromatofocusing into four isoforms: two retinol‐containing (holo‐) and two retinol‐free (apo‐) species. The pI values of the isoforms ascertained by isoelectrofocusing with an immobiline pH gradient were: holo(I)...
Nitric oxide is a diffusible messenger with multiple biological functions. We show here that NO‐generating compound, S‐nitrosoglutathione (GSNO) induces apoptosis in human chondrocytes and causes necrosis‐like celt death in human epithelial CaOv cell line. Pretreatment of chondrocytes with...
Rscu‐PA and its mutant constructed by in vitro site specific mutagenesis of Arg154 in rscu‐PA to Gly154 (mscu‐PA) were both expressed in Escherichia coli. After in vitro denaturation and renaturation, the rscu‐PA and mscu‐PA were purified to homogeneity by Zn2+ selective precipitation,...
To study the effect of several cyclins on cyclin B1 promoter activation, we co‐transfected cyclin A, cyclin E and cyclin D1 expressing plasmids with a cyclin B1 promoter construction driving a luciferase repotnter gene into NIH 3T3 cells. All three cyclins produced activation of the reporter...
Two distinct insulin‐like growth factor‐I (IGF‐1 cDNAs were isolated from a juvenile carp liver cDNA library. Both of the cDNAs encoded a full length prepro IGF‐I with 161 amino acids: a mature peptide (70 residues), its signal peptide (44 residues), and a carboxy‐terminal E domain (47...
Glutathione S‐transferase (GST) enzymes are toxicologically important from many points of view. Rat kidney derived established NRK cells were mass cultured for the isolation of GST isoenzymes. These were enriched by affinity chromatography and separated by chromatofocusing and HPLC. Exactly the...
Among the immunoreactive peptides from hamster tissues with the antibody (Ab‐1) which can recognize part of the C4 conserved region of protein kinase C (PKC), a peptide with 34 kDa (34 kDa species) is constitutively detected in the soluble and the nuclear but not in the particulate fractions....
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