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We report here the capacity of three analogs of Splenopentin, to up regulate transcription of the HLA ‐B7 gene in the K562 cell line. These cells normally do not transcribe the HLA class I genes. The Splenopentin analogs used in this study are effective at very low molar concentrations and are...
The catalytic activity of uridine phosphorylase from Escherichia coli K‐12 entrapped in hydrated reversed micelles of aerosol OT (AOT) in octane has been studied as a function of the degree of hydration of micelles. It was shown that the catalytic activity reaches maximum values at ratios...
Specificity of two different types of phospholipase A2 (PLA2) inhibitory proteins from the blood plasma of venomous snakes was investigated. Two Crotalidae inhibitors, having a carbohydrate recognition domain (CRD) in their sequences, inhibited specifically the group‐II acidic PLA2s of their...
Previous studies have reported the presence of carnitine palmitoyltransferase I and II in tumor cells and the inhibitory effects of fatty acids on cell proliferation. The present work considered the metabolic fate of (14C) or (3HI‐labeled fatty acids and their effects on cellular metabolism in...
We synthesized and used hammerhead ribozymes designed for the GUC and AUC target sequences located in the terminal loop C of animal and plant 5S rRNAs to verify its involvement in a higher order structure formation of ribosomal RNA. The activity of both GUC and AUC specific ribozymes were proven...
This study showed that nitric oxide (NO)‐generating S‐nitrosothiols, S‐nitroso‐N‐acetylpenicillamine (SNAP) and S‐nitrosoglutathione (GSNO), increased proliferation of BALB/c 3T3 (clone A31‐1‐1) fibroblasts in vitro. Treatment with SNAP at a relatively low concentration...
Leukotrienes formed by the action of 5‐lipoxygenase on arachidonic acid are a group of endogenous compounds active in hypersensitivity reactions and inflammation. In this report the formation of LTB4 from LTA4 was measured by incubating lung microsomes from normal and exercised rats to...
β‐NADH oxidase purified from Thermus aquaticus was covalently immobilised on various solid supports. The preparations obtained were compared with the soluble enzyme for activity and kinetic properties. Activated glutaryl‐PVA was found to be the best support. The immobilised enzyme was less...
Rat pancreatic islets were incubated for 90 min with L‐(4‐3H)phenylalanine in either the absence or presence of D‐glucose and D‐fructose. Relative to basal value, D‐glucose (16.7 mM) increased the incorporation of the tritiated amino acid into trichloroacetic acid‐precipitable...
To elucidate the pathogenetic mechanism of renal parenchymal injury in autosomal dominant polycystic kidney disease (ADPKD) patients, typically characterized by renal cystic changes paralleled by interstitial inflammation and gradual fibrotic changes, the role of selected inflammatory mediators...
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