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Effects of two polyanions (heparin, Nation) on absorption and CD spectra in the Soret region, and on fluorescence of ferricytochrome c were investigated. The conformational state of ferricyt c in the complexes with studied polyanions is significantly different. Whereas heparin affects the...
Claviceps fusiformis, an ergot pathogen of pearl millet, was equilibrated in media without and with 0.1 mM ouabain (g‐strophantin). Highly significant effects of ouabain on the contents of sodium ions and water were found, suggesting that an active transport of sodium ions, similar to that in...
A procedure based on CHAPS‐agarose‐polyacrylamide electrophoresis and in situ staining of activity was used to detect a Zn2+‐sensitive component of Ehrlich cell plasma membrane redox system. The procedure is so powerful that it allows to use crude plasma membrane fractions and can be easily...
An elastin‐derived peptide with an average molecular mass of 25 kDa was shown to induce monocyte chemotaxis at the optimal concentration of 10‐1 μg/ml. Homologous deactivation test showed that monocytes exposed to the elastin‐derived peptide at 10‐1 μg/ml lost their chemotactic...
The effect of the presence of protease inhibit0rs in the storage buffer on acetylcholinesterase (AChE) activity was studied in crude membrane preparations from sheep platelets before and after solubilization of the membranes with Triton X‐100. Although sensitive to the action of trypsin, the...
Streptokinase (SK) is a bacterial plasminogen activator of multi‐domain structure. In deletion analysis of the N‐terminal region of SK, the deletion of 20 amino acids (SKΔN20) resulted in the dramatic reduction of plasminogen activator activity compared to deletion of 7 (SK ΔN7) and 13...
In order to investigate the role of the α‐fragment of metallothionein (MT) in metalbinding, two mutant MTs, βAlaαCys‐ and αCysβCys‐mutant MTs, expressed in Escherichia coli were analyzed for their metal‐binding ability. A mutant MT where all of the cysteine residues in the...
Calcineurin consists of two subunits, a catalytic A subunit of 60 kDa and a regulatory B subunit of 19 kDa. Both the A and B subunits of rat brain calcineurin were expressed in E. coli. The B subunit was readily overexpressed in the pET‐21 a vector with yields of >70 mg of purified B subunit...
α‐Tocopherol, but not β‐tocopherol, negatively regulates proliferation of A7r5 vascular smooth muscle cells at physiological concentration. The HeLa cell line was not affected whereas the Chinese hamster ovary cell line (CHO) was slightly inhibited by both α‐tocopherol and...
In the present report we demonstrate the in vitro effects of free radicals on an ecto‐ATP diphosphohydrolase activity (apyrase, EC 126.96.36.199) from rat blood platelets. Rat blood platelets were exposed to an oxidant‐generating system (H202 / Fe2+ / ascorbate) and the ATP diphosphohydrolase...
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