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Summary NHIK 3025 cells were synchronized by repeated mitotic selection. The S-phase was determined by 3 H-thymidine incorporation and scintillation counting. By comparing the age–response curves of aerobic cells irradiated with 500 rad with those of extremely hypoxic (< 4 p.p.m. O 2 ) cells...
Summary The chymotrypsin-like proteins (chymotrypsin-CT, chymotrypsinogen-CTG, trypsin-T and modified chymotrypsins-at Met 192-MCT and at Tyr 146, 171-TCT), γ-irradiated in the presence of air, were investigated. Irradiation leads to the unfolding of the native structure of CT-like proteins both...
Summary The uptake of radioactive 5′-dTMP into the DNA of diploid yeast cells was measured in the G 1 and S-phase of the cell-cycle. In control cells, the uptake is zero in G 1 and increases with time in the S-phase. Cells irradiated in early G 1 show an uptake (unscheduled DNA synthesis) which...
Summary EMT6 mouse mammary tumour cells in vitro were exposed to an almost uniform 1400-gauss magnetic field during or after irradiation with 120 kV X-rays. Exposure of unirradiated control cultures to this field for up to 48 hours did not alter the viability or growth of the cells. Exposure of...
Summary Electron paramagnetic resonance (e.p.r.) was used to study the free radicals induced by γ-irradiation at 77 K in oriented DNA with incorporated electron-affinic radiosensitizing compounds (4-nitroacetophenone, metronidazole, and Ro-07-0582). The observed e.p.r. spectra were compared with...
Summary The catabolism of uracil and the total balance of excreted radioactivity were studied in golden hamsters after a peroral application of 14 C-uracil. Twenty-four hours after administration most of the radioactivity taken up appeared in expired carbon dioxide. The percent proportion of...
Summary The oxygen-dependent damage which develops in barley seeds with ∼7·8 per cent moisture content disappears after post-irradiation storage in vacuo for 48 hours at 40°C and for 24 hours at 50°C. When the duration of storage at 40°C is extended to 384 hours, oxygen-independent damage...
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