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Although much of productive research has been conducted in the field of molecular virology of Hepatitis C virus (HCV) regarding its genes, gene functions and proteins, development of an efficient cell culture model for its replication remained a focused area. Focus has been directed to establish...
Highlights ► We compare cagA EPIYA genotypes in gastric DNA and cultured Helicobacter pylori strain DNA. ► An improved PCR-based method allowed for high throughput cagA EPIYA genotyping. ► The improved method rapidly detects mixed H. pylori strain infections. ► Genotyping should be...
Highlights ► Microsatellite typing of Leishmania donovani complex isolates discriminates intercontinental groups. ► Genome-wide SNP profiling reveals diversity in a homogeneous population. ► Identification of a novel divergent lineage within a small geographic region. ► SNP-typing of...
Highlights ► We validate PCR cytochrome b heteroduplex assay to identify blood meals in triatomines. ► Blood meals detection provides information on triatomine movements among ecotopes. ► This method combined with sequencing is a useful tool in epidemiological studies.
Highlights ► Intrahost passage alters acid resistance and cell-associated kinetics of Listeria monocytogenes . ► The altered phenotypes link to the SigB activity. ► Themutation in rsbW locus (a regulator for SigB protein) is assumed to be a major cause for the phenotypic alterations.
Highlights ► HPV 53 isolates from 15 Brazilian women were analyzed to detect genomic variants. ► L1, E6 and E7 genomic fragments were sequenced to identify nucleotide changes. ► HPV 53 genetic polymorphism was characterized based on L1 sequences. ► One co-infected L1 and two new E6...
Highlights ► GBV-C ORF, and subgenomic sequence segments were analysed. ► Phylogenetic noise is determined by the sequence size. ► Most regions of the viral genome seem to evolve under the same model assumptions. ► Dispersion of informative sites across the viral coding sequence was...
Highlights ► A cowpox virus from a human patient in Norway produced atypical A-type inclusions. ► The atip gene of the cowpox virus is identical to that of ectromelia virus. ► Recombination event occurred at the beginning of the atip gene.
Highlights ► The circulating dengue viruses in Singapore are highly diverse. ► Multiple importations and in situ evolution contribute to viral diversity in Singapore. ► In situ evolution could possibly result in viruses with improved fitness.
Highlights ► Presence of Mycobacterium leprae in the environment by using PCR targeting 16S rRNA. ► Viable M. leprae were found in the areas where active leprosy patients reside. ► Similar genotype of M. leprae found in soil and patients residing in the same area. ► This could help in...
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