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Patients infected with HIV-1 frequently have a sensory neuropathy, but the cause is unknown. We found that the gp120 glycoprotein of HIV-1 bound to the surface of cultured rat dorsal root ganglia (DRG) neurons, and activated the complement cascade to lyse the neuronal cells. Cytotoxicity was...
An ovine cosmid library was screened with a bovine Cϵ gene probe. IgE and IgA constant region genes (Cϵ and C α , respectively) were isolated within a single 26 kb cosmid insert. The Cϵ gene was cloned into an expression vector which contained the rearranged VDJ genomic segment encoding an...
Patients with cystic fibrosis (CF) have a high prevalence of Pseudomonas aeruginosa infection which causes chronic infection of the mucosal surfaces of the lung. This results in recurrent immune stimulation and hypergamma-globulinemia. The present study examines the levels of circulating Ig...
The speculation that immunologically reactive haptens must be those attached to carriers' immunodominant epitopes suggests a clearer mechanism by which the mysterious hapten-carrier phenomena are generated. This review focuses on the molecular biological nature of immune recognition of...
Monoclonal antibodies (MAbs) against IgG3 allotypic markers were evaluated in haemagglutination inhibition and IgG3 capture ELISA. MAbs PNF69C and 200D1 exhibited G3m(u) and G3m(g) specificity respectively. In HAI target epitopes detected by MAbs were remarkably stable to physiochemical...
We employed anti-human sperm monoclonal antibodies to investigate how sperm membrane antigens are involved in gamete interactions. We have produced seven monoclonal antibodies specific for human sperm antigens, that showed reaction with mouse sperm by ELISA and by immunofluorescence. These...
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