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Rabbits immunized with purified Sm and RNP small nuclear ribonucleo-proteins (snRNPs) produced precipitating and hemagglutinating antibodies against these antigens. These antibodies had immunological specificity identical to the naturally occurring SLE anti-Sm/RNP antibodies as demonstrated by...
In the present experiment we attempted to confirm the specific stimulation of substance P (SP) on human T lymphocyte proliferation as reported by Payan et al. and to observe its effect on human B lymphocyte proliferation. As a result, no definite mitogenic effect of SP (10 −11 to 10 −7 M) was...
Circulating immune complexes were detected by the immunoelectrophoretic method in 18 of 29 (62 per cent) of patients with systemic scleroderma. The presence of immune complexes did not correlate with that of antinuclear antibodies to dsDNA, DNP, RNP, and Sm. The mean levels of immunoglobulins G,...
A spin-membrane immunoassay has been employed to examine the immune reactivity of whole serum from patients with chronic-progressive multiple sclerosis (CPMS) against liposomes containing ganglioside GM 1 . Exposure to serum resulted in complement-mediated lysis of the GM 1 -liposomes. No lysis...
The protein A-binding site of human IgM was studied by affinity chromatography on SpA-Sepharose using fragments derived from a human monoclonal SpA-reactive IgM, Iz. Neither Fabμ nor (Fc)5μ fragments were retained on the column but IgM reactivity was unaffected by thermic treatment during...
We have observed that CT6 cell line, a murine interleukin 2 (IL 2)-dependent T cell line, was highly responsive to phorbol myristate acetate (PMA) and to a lesser extent but significantly responsive to lipopolysaccharide (LPS) in the short-term proliferation assay. In contrast, two other murine...
Endogenous immunoglobulin (Ig) determinants on blood B-lymphocytes (B-cells) were investigated in 13 healthy individuals, 9 patients with thyrotoxic Graves disease, 5 patients with chronic sarcoidosis, and 4 patients with IgA deposition in renal glomeruli. Specificities of goat antisera to Ig...
Infectious mononucleosis sera gave positive results in enzymoimmunoassay with glutaraldehyde-treated human erythrocytes. This unexpected reaction appeared to be caused by the interaction of Paul-Bunnell (P-B) antibodies with a partial P-B antigen that apparently appears on human red blood cells...
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