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Previous studies from this laboratory have resulted in the determination of the entire antigenic structure of myoglobin (Mb). The present work was carried out to investigate the antigenicity of the svnthetic antigenic sites of Mb in their free form (i.e. without coupling to a carrier) and the...
Negatively charged multilamellar liposomes, covalently coupled with p-aminophenylated derivative of D-galactose, have been used to raise galactose specific antibodies in rabbits. The specificity of the antiserum has been characterized hy immunociffusion, quantitative precipitation and hapten...
Experimental allergic encephalomyelitis (EAE) is an experimental, T-cell dependent, autoimmune disease of the central nervous system that can be induced in most mammals by the injection of myelinated neural tissue in complete Freund's adjuvant. Relatively small doses, 10 4.6 Units/kg, of rat...
The extent of binding of anti-D antibody to intact Rh (D) positive human erythrocytes at -2.5° was approximately one-third that at.3-°. An Arrhenius plot of the temperature dependence of antibody binding showed a clear and reproducible discontinuity at approximately 6-8°. Phospholipasc A 2...
Highly purified human factor B of the alternative complement pathway was treated with factor D in the presence of cobra venom factor to generate its Ba and Bb cleavage fragments. These cleavage fragments were isolated by preparative polyacrylamide gradient gel electrophoresis followed by...
Serum obtained from patients with spontaneous systemic lupus erythematosus or from patients treated at least 3 months with procainamide could specifically inhibit Con A mitogenesis of cultured peripheral blood mononuclear cells obtained from procainamide-treated patients or from normal donors....
Specific anti-rabbit acrosin antibodies were prepared by immunizing female guinea pigs with rabbit acrosin. Acrosin was purified by molecular sieve and proflavin-Sepharose affinity chromatography. Inhibition studies to characterize unique antigenic determinants of acrosin compared to pancreatic...
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