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Sodium metaperiodate stimulates human small lymphocytes to transform into blast cells which synthesize DNA. The triggering of this lymphocyte activation occurs within minutes, but can be blocked by pre-treatment of the periodate with reducing agents. The inhibition of the periodate effect on...
51 Chromim release micro-assays for both lymphocyte and antibody cytotoxicity have been developed. Tests vs. several different types of target cells can be performed on 1 to 5 ml of blood. The results are objective, highly reproducible and can be obtained within 6 hrs. Application of this test...
Treatment of mice with high sublethal doses of cholera entero-exotoxin, or chol-eragen, given i.v. on days 4 and 6 post-coitum , i.e., around the time of nidation, decreases or suppresses pregnancy in females of 3 different strains of mice. Our studies reveal that the toxin interferes with...
In the guinea pig, delayed administration of complete Freund's adjuvant (CFA) to skin sites previously primed with a cellular antigen leads to a marked intensification of the acquired sensitivity to that antigen. This effect has been demonstrated with diverse cellular antigens including foreign...
Hapten binding sites of chicken anti-DNP antibody appear to contain an iodinatable residue since their binding activity is decreased by iodi-nation of the antibody. The presence of the hapten -DNP-lysine during iodination protects the site. Peptides were isolated from peptic digests of the...
An improved migration inhibitory factor (MIF) assay procedure is described which was developed for the study of tumor patient immunocompetence testing both on the clinical level and as an adjunct to basic studies of tumor immunology and the transfer of immunological capacity through immuno-genic...
The developnent of delayed hypersensitivity as measured by footpad swelling follawing immunization with erythrocyte antigen closely parallels the development of the radioresistant, carrier erythrocyte specific thymus-derived cell population of the spleen capable of enhancing the anti-hapten...
The capacity to produce antibodies to fetal antigens in syngeneic animals has been established. Fetal intestine from inbred Lewis rats injected into adult rats of the same strain provoked fetal intestine-specific antibodies in the serum as judged by immuno-fluorescence. In addition,...
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