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We have studied the mutual effects of chloride ion and EDTA on the dimer-tetramer assembly of human deoxy-hemoglobin and oxyhemoglobin. It is found that these two anions have similar but interdependent effects. In low Cl − (.01 M) increasing concentrations of EDTA are found to decrease both...
Topical Reviews in Haematology - Volume 2, edited by S. Roath. London, Bristol, Roston, Wright/PSG, 1982. 222 pp. Price: $32.50.
We investigated hemoglobin synthesis in suspension cultures of liver erythroid cells obtained from calf fetuses of 103 to 198 days. A significant amount of radioactivity was associated with adult hemoglobins which were separated from the fetal hemoglobins by isoelectric focusing, even after...
DNA was isolated from the white cells of a subject with the Hb S-Kenya-Gγ-HPFH condition, and digested with the restriction endo-nucleases Bgl II, Xba I, and Eco RI. The resulting fragments were identified by standard techniques using ( 32 P) labelled probes which are specific for the second...
Hemoglobin Boyle Heights (α6 (A4) Asp → 0) is the first observed variant with a deletion in the α chain. The variant, which was detected in a 39-year-old man of Mexican descent, constitutes 14% of the total hemoglobin and is associated with microcytosis, heat instability, and increased oxygen...
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